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Attachment of Cell Walls of Chlamydia psittaci to Mouse Fibroblasts (L Cells)

机译：鹦鹉热衣原体细胞壁对小鼠成纤维细胞（L细胞）的附着

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14C-labeled cell walls of the 6BC strain of Chlamydia psittaci, prepared from intrinsically labeled chlamydial cells by digestion with deoxycholate and trypsin, associated with mouse fibroblasts (L cells) in a manner comparable to that of intact C. psittaci. Almost half of the host cell-associated cell walls were not dissociated by trypsin, suggesting that they had been attached and then ingested. The attachment of cell walls to L cells was inhibited by a number of treatments known to block association of intact C. psittaci with L cells: heating the cell walls for 3 min or reacting them with antiserum against intact C. psittaci, or pretreating the L cells with trypsin or wheat germ agglutinin. Unlike intact cells of C. psittaci, cell walls were not immediately toxic for L cells, and they did not measurably adsorb neutralizing antibody. As revealed by making cell walls from intact C. psittaci labeled with 125I by lactoperoxidase-catalyzed iodination, cell walls contained a much smaller number of surface-labeled proteins than did whole chlamydial cells. The most abundant surface-labeled protein was one with an apparent molecular weight of 43,000. In the final step of cell wall preparation, tryptic digestion of deoxycholate-extracted cells, this major surface protein was partially cleaved to a 40,000-dalton product. When the major surface protein (both the 43,000- and 40,000-dalton moieties) was electrophoretically separated from the other cell wall proteins and used to immunize a rabbit, antibodies that neutralized the infectivity of intact C. psittaci were elicited. It was concluded that cell walls retain the ability to associate with L cells in much the same way as do intact cells of C. psittaci, but, despite the simpler structure of cell walls, the element that binds C. psittaci to host cells cannot yet be identified.
机译：14 C标记的衣原体衣原体的6BC菌株的细胞壁，是通过固有地标记的衣原体细胞经脱氧胆酸盐和胰蛋白酶消化而制备的，并与小鼠成纤维细胞（L细胞）相关。与完整 C相当的方式。宿主细胞相关的细胞壁几乎没有被胰蛋白酶解离，表明它们已经被附着并被摄入。细胞壁对L细胞的附着被许多已知阻断完整C缔合的处理所抑制。 psittaci （带有L个细胞）：加热细胞壁3分钟，或使它们与抗 C的抗血清反应。 psittaci ，或用胰蛋白酶或小麦胚芽凝集素预处理L细胞。与完整的 C细胞不同。 psittaci ，细胞壁对L细胞没有立即毒性，并且没有可测量地吸附中和抗体。正如通过完整的 C制作细胞壁所揭示的。通过乳过氧化物酶催化的碘化作用以 125 I标记的psittaci ，与整个衣原体细胞相比，细胞壁所含的表面标记蛋白数量要少得多。表面标记的蛋白质最多，是一种表观分子量为43,000的蛋白质。在细胞壁制备的最后一步，即脱氧胆酸盐提取细胞的胰蛋白酶消化中，该主要表面蛋白被部分切割成40,000道尔顿的产物。当将主要表面蛋白（43,000-40,000道尔顿和40,000道尔顿的部分）与其他细胞壁蛋白电泳分离并用于免疫兔时，抗体会中和完整C的感染性。引发了psittaci 。得出的结论是，细胞壁保留与L细胞缔合的能力，与完整的C细胞一样。 psittaci ，但是，尽管细胞壁的结构更简单，但绑定 C的元素。 psittaci 宿主细胞尚无法确定。

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