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首页> 外文期刊>Infection and immunity >Alterations in the equine herpesvirus 1 genome after in vitro and in vivo virus passage.
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Alterations in the equine herpesvirus 1 genome after in vitro and in vivo virus passage.

机译：体外和体内病毒通过后马疱疹病毒1基因组的变化。

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The effect of in vitro and in vivo serial virus passage on the genetic stability of equine herpesvirus 1 (EHV-1) was investigated by restriction endonuclease analysis of the viral DNA. DNAs of EHV-1 isolates at different passage levels in cultured cells or in Syrian hamsters were compared by electrophoresis of the DNA cleavage fragments produced by restriction endonuclease digestion. No changes were observed in the restriction profile of the DNAs of EHV-1 strains after 100 sequential passages in cultured equine cells. However, serial passage of the virus in hamsters or in cells of non-equine origin quickly gave rise to alterations in the viral DNA. These changes occurring in the restriction endonuclease profiles of EHV-1 DNA during serial virus passage in non-equine cells or animals hosts could be explained by sequence additions or deletions to preexisting restriction fragments resulting in variation in their electrophoretic mobilities.
机译：通过对病毒DNA进行限制性核酸内切酶分析，研究了体内和体外系列病毒传代对马疱疹病毒1（EHV-1）遗传稳定性的影响。通过电泳分析限制性核酸内切酶消化产生的DNA裂解片段，比较培养细胞或叙利亚仓鼠中EHV-1分离株在不同传代水平下的DNA。在培养的马细胞中连续传代100次后，EHV-1菌株DNA的限制性酶切图谱未见变化。但是，病毒在仓鼠或非马源细胞中的连续传代很快引起病毒DNA的改变。 EHV-1 DNA的限制性核酸内切酶谱在非马匹细胞或动物宿主中进行连续病毒传代过程中发生的这些变化，可以通过对先前存在的限制性片段进行序列添加或缺失来导致其电泳迁移率变化来解释。

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