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首页> 外文期刊>Infection and immunity >Association of neuraminidase-sensitive receptors and putative hydrophobic interactions with high-affinity binding sites for Streptococcus sanguis C5 in salivary pellicles.
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Association of neuraminidase-sensitive receptors and putative hydrophobic interactions with high-affinity binding sites for Streptococcus sanguis C5 in salivary pellicles.

机译：神经唾液酸酶敏感受体和推定疏水性相互作用与唾液薄膜中血红链球菌C5高亲和力结合位点的关联。

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摘要

The relationship of neuraminidase-sensitive receptors and putative hydrophobic interactions to high- and low-affinity binding sites in experimental salivary pellicles for Streptococcus sanguis C5 was investigated. NaSCN, an inhibitor of hydrophobic interactions, reduced the number of cells which adsorbed to pellicles to a greater extent than NaCl or KCl when both low and high streptococcal concentrations were used in assays. However, NaSCN was not more effective than NaCl or KCl in desorbing 3H-labeled salivary pellicle components from hydroxyapatite, and NaSCN pretreatment of either strain C5 cells or the salivary pellicles did not destroy or remove either the streptococcal adhesions or the pellicle receptors. Neuraminidase treatment of pellicles or the presence of sialic acid-containing gangliosides only inhibited S. sanguis adsorption when low streptococcal concentrations were used. At these concentrations, S. sanguis adsorbs primarily to high-affinity pellicle binding sites. Adsorption isotherms indicated that neuraminidase-sensitive interactions were mainly responsible for the high affinity of these binding sites, whereas putative hydrophobic interactions inhibitable by NaSCN were mainly associated with the numbers of binding sites available. Sugar inhibition studies suggested that the two classes of binding sites previously demonstrated in untreated salivary pellicles for S. sanguis C5 are not the result of a partial conversion of high-affinity sites to low-affinity sites due to removal of sialic acid residues.
机译：研究了血尿链球菌C5实验唾液薄膜中神经氨酸酶敏感受体和假定的疏水相互作用与高亲和力和低亲和力结合位点之间的关系。当在测定中同时使用低浓度和高浓度的链球菌时，NaSCN是疏水性相互作用的抑制剂，与NaCl或KCl相比，减少了吸附至薄膜的细胞数量。但是，NaSCN在从羟基磷灰石上解吸3H标记的唾液薄膜组件方面并不比NaCl或KCl更有效，并且NaSCN对C5菌株或唾液薄膜组件的预处理不会破坏或去除链球菌粘附或薄膜组件受体。当使用低链球菌浓度时，膜的神经氨酸酶处理膜或含有唾液酸的神经节苷脂仅抑制血红链霉菌的吸附。在这些浓度下，血红链球菌主要吸附至高亲和力的防护膜结合位点。吸附等温线表明神经氨酸酶敏感的相互作用主要负责这些结合位点的高亲和力，而被NaSCN抑制的推定疏水相互作用则主要与可用结合位点的数量有关。糖抑制研究表明，先前在未经处理的唾液薄膜中证明的两类结合位点不是由于唾液酸残基去除而将高亲和力部分转化为低亲和力的结果。

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