Antimicrobial binding of a radiolabeled cationic neutrophil granule protein.

M M Farley; W M Shafer; J K Spitznagel

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Antimicrobial binding of a radiolabeled cationic neutrophil granule protein.

机译：放射性标记的阳离子中性粒细胞颗粒蛋白的抗菌结合。

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A 57,000-dalton protein (CAP57) purified from human polymorphonuclear leukocytes has antimicrobial activity against a number of gram-negative bacteria. We developed a procedure using solid-phase Iodo-gen to radiolabel CAP57 without destroying its antibacterial activity. Iodinated and native CAP57 were electrophoretically identical. Autoradiographs of sodium dodecyl sulfate-polyacrylamide gels revealed greater than 95% of the 125I in a single heavy band in the 57,000-molecular-weight region. The quantity of [125I]CAP57 bound to bacterial test strains was directly proportional to the sensitivity to CAP57.

机译：从人多形核白细胞中纯化的57,000道尔顿蛋白质（CAP57）对许多革兰氏阴性细菌具有抗菌活性。我们开发了一种使用固相碘原对CAP57进行放射性标记而不破坏其抗菌活性的方法。碘化的和天然的CAP57在电泳上是相同的。十二烷基硫酸钠-聚丙烯酰胺凝胶的放射自显影照片显示，在57,000分子量区域的单个重链中，125I的95％以上。与细菌测试菌株结合的[125I] CAP57的数量与对CAP57的敏感性成正比。

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《Infection and immunity》 |1987年第6期|共4页
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M M Farley; W M Shafer; J K Spitznagel;
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1. Lipopolysaccharide structure determines ionic and hydrophobic binding of a cationic antimicrobial neutrophil granule protein. [J] . M M Farley, W M Shafer, J K Spitznagel Infection and immunity . 1988,第6期

机译：脂多糖结构决定阳离子抗菌中性粒细胞颗粒蛋白的离子和疏水结合。
2. Cutting edge: cationic antimicrobial peptides block the binding of lipopolysaccharide (LPS) to LPS binding protein. [J] . Scott MG, Vreugdenhil AC, Buurman WA, The Journal of Immunology: Official Journal of the American Association of Immunologists . 2000,第2期

机译：前沿：阳离子抗菌肽可阻断脂多糖（LPS）与LPS结合蛋白的结合。
3. Late intraphagosomal hydrogen ion concentration favors the in vitro antimicrobial capacity of a 37-kilodalton cationic granule protein of human neutrophil granulocytes. [J] . W M Shafer, L E Martin, J K Spitznagel Infection and immunity . 1986,第3期

机译：晚期吞噬体内氢离子浓度有利于人类嗜中性粒细胞的37千达尔顿阳离子颗粒蛋白的体外抗菌能力。
4. Insights into the Protein Binding of Short Cationic Antimicrobial Peptides [C] . Johan Svenson, Bjorn-Olav Brandsdal, Wenche Stensen International Peptide Symposium . 2009

机译：洞察短阳离子抗菌肽蛋白质结合
5. Mouse cortical granules: Biochemical heterogeneity, migration, pre-fertilization release, and identification of a novel cortical granule protein. [D] . Liu, Min. 2004

机译：小鼠皮质颗粒：生化异质性，迁移，受精前释放和新型皮质颗粒蛋白的鉴定。
6. Antimicrobial binding of a radiolabeled cationic neutrophil granule protein. [O] . M M Farley, W M Shafer, J K Spitznagel 1987

机译：放射性标记的阳离子中性粒细胞颗粒蛋白的抗菌结合。
7. Synthetic bactericidal peptide based on CAP37: a 37-kDa human neutrophil granule-associated cationic antimicrobial protein chemotactic for monocytes. [O] . Pereira, H A, Erdem, I, Pohl, J, 1993

机译：基于CAP37的合成杀菌肽：单核细胞的37 kDa人类嗜中性粒细胞相关阳离子阳离子抗菌蛋白。

Antimicrobial binding of a radiolabeled cationic neutrophil granule protein.

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