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首页> 外文期刊>Infection and immunity >Antibody responses against Mycobacterium tuberculosis in 11 strains of inbred mice: novel monoclonal antibody specificities generated by fusions, using spleens from BALB.B10 and CBA/J mice.
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Antibody responses against Mycobacterium tuberculosis in 11 strains of inbred mice: novel monoclonal antibody specificities generated by fusions, using spleens from BALB.B10 and CBA/J mice.

机译：11种近交小鼠对结核分枝杆菌的抗体应答：使用BALB.B10和CBA / J小鼠的脾脏，融合产生的新型单克隆抗体特异性。

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Eleven strains of inbred mice were immunized with a culture filtrate of Mycobacterium tuberculosis H37Rv, and the quality of the antibody responses was determined by immunoblotting. The quantity of mycobacterial antigen used for each immunization ranged from 6 to 750 micrograms per inoculum. The culture filtrate of M. tuberculosis was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrotransferred to nitrocellulose filters. Immunoblotting results were obtained with serum from the following 11 strains of immunized mice: C57BL/6J, BALB/cJ, BALB.B10, C3H.OH, A.CA/Sn, CBA/J, DBA/1J, DBA/2J, C3H/HeJ, B10.A/SgSn, and B10.D2Sn. Mice were tested individually, and results from each mouse were compared after each immunization. It was found that sera from individual mice within the same strain differed only slightly in their immune response patterns. In contrast, major differences were seen when the reactivities of sera from different strains were compared. Hybridomas were obtained from cell fusions by using spleen cells from BALB.B10 and CBA/J mice. Twelve monoclonal antibodies were raised, which identified epitopes on molecules with different electrophoretic mobilities than those already described by other investigators. The monoclonal antibodies were characterized by immunoblotting with respect to their reactivities with culture filtrates from M. tuberculosis and six other mycobacterial species. One of the monoclonal antibodies (HBT-10) identified an epitope that was present in M. tuberculosis H37Rv but not in Mycobacterium bovis BCG.
机译：用结核分枝杆菌H37Rv的培养滤液对11个近交系小鼠进行免疫，并通过免疫印迹法确定抗体反应的质量。每次免疫使用的分枝杆菌抗原的量为每个接种物6到750微克。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离结核分枝杆菌的培养滤液，并将其电转移到硝酸纤维素滤膜上。使用以下11种免疫小鼠品系的血清获得了免疫印迹结果：C57BL / 6J，BALB / cJ，BALB.B10，C3H.OH，A.CA / Sn，CBA / J，DBA / 1J，DBA / 2J，C3H /HeJ、B10.A/SgSn和B10.D2 / nSn。分别对小鼠进行测试，并在每次免疫后比较每只小鼠的结果。已经发现，来自同一株内的个别小鼠的血清在其免疫应答模式上仅稍有不同。相反，当比较来自不同菌株的血清的反应性时，看到了主要差异。通过使用BALB.B10和CBA / J小鼠的脾细胞从细胞融合物中获得杂交瘤。提出了十二种单克隆抗体，该抗体识别了与其他研究人员已经描述过的电泳迁移率不同的分子上的表位。通过与来自结核分枝杆菌和其他六个分枝杆菌属的培养滤液的反应性进行免疫印迹来表征单克隆抗体。单克隆抗体之一（HBT-10）鉴定了一个存在于结核分枝杆菌H37Rv中但不存在于牛分枝杆菌BCG中的表位。

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