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首页> 外文期刊>Infection and immunity >Fibronectin increases Trypanosoma cruzi amastigote binding to and uptake by murine macrophages and human monocytes.
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Fibronectin increases Trypanosoma cruzi amastigote binding to and uptake by murine macrophages and human monocytes.

机译：纤连蛋白可增加克氏锥虫假单胞菌与鼠巨噬细胞和人单核细胞的结合并被其吸收。

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Trypanosoma cruzi amastigotes present receptors for human fibronectin as indicated by the saturable binding of [125I]fibronectin to this form of the parasite. Scatchard analysis indicates that the number of fibronectin receptors per amastigote was 1.3 x 10(3) with a Kd of approximately 2.3 nM. Addition of physiological concentrations of fibronectin to amastigote-macrophage cocultures significantly increased the binding of amastigotes to murine macrophages. This increase was evidenced in both the number of amastigotes bound to macrophages and the percentage of macrophages containing bound amastigotes. The uptake of amastigotes by either murine macrophages or human blood monocytes was also increased in the presence of exogenous fibronectin. The increase induced by fibronectin was blocked when amastigotes were pretreated with the RGDS tetrapeptide of the fibronectin cell attachment site. Furthermore, the ability of fibronectin to enhance amastigote binding to and uptake by macrophages was inhibited by the F(ab')2 fragment of anti-fibronectin immunoglobulin G (IgG) but not by an irrelevant anti-human IgG F(ab')2 fragment. Pretreatment of either amastigotes or macrophages with fibronectin also resulted in a significant increase in amastigote binding to and uptake by macrophages. These results suggest that fibronectin may play a role in facilitating the attachment and ingestion of T. cruzi amastigotes by macrophages and monocytes in chagasic tissue lesions.
机译：如[125I]纤连蛋白与这种形式的寄生虫的可饱和结合所表明的，克鲁氏锥虫锥虫存在人类纤连蛋白的受体。斯卡查德（Scatchard）分析表明，每个假肢体纤连蛋白受体的数量为1.3 x 10（3），Kd约为2.3 nM。将生理浓度的纤连蛋白添加到吻合口巨噬细胞共培养物中显着增加了变形虫与鼠巨噬细胞的结合。结合至巨噬细胞的变形虫的数量和含有结合的变形虫的巨噬细胞的百分比都证明了这种增加。在存在外源性纤连蛋白的情况下，鼠巨噬细胞或人血单核细胞对变形虫的摄取也增加了。当用纤连蛋白细胞附着位点的RGDS四肽预处理变形虫时，纤连蛋白诱导的增加被阻断。此外，抗纤连蛋白免疫球蛋白G（IgG）的F（ab'）2片段抑制纤连蛋白增强与鞭毛蛋白结合并被巨噬细胞摄取的能力，但不受无关的抗人IgG F（ab'）2抑制。分段。用纤连蛋白对变形虫或巨噬细胞进行预处理也导致a蛇毒与巨噬细胞的结合和摄取的显着增加。这些结果表明纤连蛋白可能在促进chagasic组织病变中巨噬细胞和单核细胞对克鲁斯氏吻合动物的附着和摄取中发挥作用。

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