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首页> 外文期刊>Infection and immunity >Movement of a falciparum malaria protein through the erythrocyte cytoplasm to the erythrocyte membrane is associated with lysis of the erythrocyte and release of gametes.
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Movement of a falciparum malaria protein through the erythrocyte cytoplasm to the erythrocyte membrane is associated with lysis of the erythrocyte and release of gametes.

机译：恶性疟疾蛋白通过红细胞胞质到达红细胞膜的运动与红细胞的溶解和配子的释放有关。

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摘要

Erythrocytes containing mature gametocytes of Plasmodium falciparum circulate in the blood until they are ingested by a mosquito, an event that triggers gametogenesis and lysis of the infected erythrocyte. It was previously shown that a parasite protein (Pf155/RESA) accumulates in the erythrocyte cytoplasm next to the parasitophorous vacuolar membrane (S. Uni, A. Masuda, M. J. Stewart, R. Nussenzweig, and M. Aikawa, Am. J. Trop. Med. Hyg., 36:481-488, 1987). Using a monoclonal antibody to Pf155/RESA and rabbit sera to two different repeat peptides of Pf155/RESA, we have studied the location of Pf155/RESA after induction of gametogenesis. Five minutes after triggering gametogenesis, the parasitophorous membrane no longer surrounded the parasite, bringing the parasite membrane in contact with the erythrocyte cytoplasm. Clear spaces appeared throughout the hemoglobin-rich host cytoplasm; Pf155/RESA was now localized in the cytoplasm directly surrounding the spaces. No membrane existed between the spaces and the erythrocyte cytoplasm. The spaces with surrounding Pf155/RESA protein extended to the erythrocyte membrane. After lysis of the erythrocyte membrane (15 min after triggering gametogenesis), the protein was distributed along the erythrocyte membrane and throughout the space between the gamete and the erythrocyte membrane. The mechanism by which Pf155/RESA remained aggregated around the spaces and its role in erythrocyte lysis are unknown. It is of interest that the parasite appeared to use the same molecule during invasion of erythrocytes and during release of gametes from infected erythrocytes.
机译：含有恶性疟原虫成熟配子细胞的红细胞在血液中循环直至被蚊子摄入为止，该事件触发配子发生和被感染红细胞的溶解。先前已显示，寄生虫蛋白（Pf155 / RESA）聚集在红细胞液泡膜旁边的红细胞液泡膜（S. Uni，A。Masuda，MJ Stewart，R。Nussenzweig和M. Aikawa，Am。J. Trop） （Hyd.Med.Hyd。，36：481-488，1987）。使用针对Pf155 / RESA的单克隆抗体和针对Pf155 / RESA的两个不同重复肽的兔血清，我们研究了配子发生后Pf155 / RESA的位置。触发配子发生后五分钟，寄生虫的膜不再包围寄生虫，使寄生虫的膜与红细胞的细胞质接触。整个富含血红蛋白的宿主细胞质中都出现了清晰的空间。 Pf155 / RESA现在位于直接围绕空间的细胞质中。间隙与红细胞胞质之间不存在膜。具有周围Pf155 / RESA蛋白的空间延伸到红细胞膜。裂解红细胞膜后（触发配子发生后15分钟），蛋白质沿红细胞膜分布并遍及配子和红细胞膜之间的空间。 Pf155 / RESA保持聚集在空间周围的机制及其在红细胞溶解中的作用尚不清楚。令人感兴趣的是，该寄生虫在红细胞入侵期间以及从受感染的红细胞释放配子期间似乎使用相同的分子。

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