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首页> 外文期刊>Infection and immunity >Genetic linkage among cloned genes of Streptococcus mutans.
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Genetic linkage among cloned genes of Streptococcus mutans.

机译：变异链球菌的克隆基因之间的遗传连锁。

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Mapping vectors containing antibiotic resistance markers inserted adjacent to or within different cloned genes from Streptococcus mutans were used to determine the relative positions of these genes on the chromosome. The gtfA, ftf, and scrB genes were inserted into streptococcal mapping vector pVA891 adjacent to an Emr gene, whereas the Emr marker was inserted directly into the gtfB gene. These chimeric plasmids were transformed into S. mutans GS-5, selecting for Emr transformants. To determine the positions of the cloned genes relative to each other, it was necessary to construct plasmids labeled with a different antibiotic resistance marker. Thus, a Tetr gene was inserted adjacent to gtfB in the appropriate mapping vector and within the ftf and scrB genes with a mini-Mu transposon (Mu dT). The chimeric plasmids were transformed into the appropriate Emr recipients, and the DNA from the resulting Emr Tetr transformants was used in linkage studies. Based on the cotransfer data, gtfB was not closely linked to gtfA, ftf, or scrB. However, gtfA cotransferred with ftf and scrB at frequencies of approximately 96 and 80%, respectively. The percent cotransfer of ftf and scrB was approximately 92. These data indicate that the three genes are clustered on the GS-5 chromosome, with ftf located between gtfA and scrB. Little, if any, linkage was observed between these genes and a variety of other random markers.
机译：使用含有插入到来自变形链球菌的不同克隆基因附近或之内的抗生素抗性标记的作图载体来确定这些基因在染色体上的相对位置。将gtfA，ftf和scrB基因插入与Emr基因相邻的链球菌作图载体pVA891，而将Emr标记物直接插入gtfB基因。将这些嵌合质粒转化到变形链球菌GS-5中，选择Emr转化体。为了确定克隆的基因彼此之间的位置，有必要构建用不同抗生素抗性标记物标记的质粒。因此，在合适的作图载体中，将gtrB附近的Tetr基因插入，并用mini-Mu转座子（Mu dT）将ftf和scrB基因插入。将嵌合质粒转化到合适的Emr受体中，并将​​所得Emr Tetr转化子的DNA用于连锁研究。根据共转移数据，gtfB与gtfA，ftf或scrB没有紧密联系。但是，gtfA与ftf和scrB分别以大约96％和80％的频率共转移。 ftf和scrB的共转移百分比约为92。这些数据表明这三个基因聚集在GS-5染色体上，而ftf位于gtfA和scrB之间。这些基因与各种其他随机标记之间几乎没有联系，甚至没有联系。

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