• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Immune T cells sorted by flow cytometry confer protection against infection with Treponema pallidum subsp. pertenue in hamsters.
【24h】

Immune T cells sorted by flow cytometry confer protection against infection with Treponema pallidum subsp. pertenue in hamsters.

机译：通过流式细胞仪分选的免疫T细胞可预防梅毒螺旋体亚种感染。仓鼠的持久性。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The role of cell-mediated immunity against infection with Treponema pallidum subsp. pertenue in humans or experimental animals is unclear. Hamsters injected subcutaneously in the hind paws with 4 x 10(6) unfractionated lymph node cells or enriched lymph node T cells (immunoglobulin negative, Ia negative) from T. pallidum subsp. pertenue-immune hamsters were resistant to challenge with T. pallidum subsp. pertenue. The popliteal lymph nodes of hamsters that received immune cells weighed less and had significantly fewer treponemes than did lymph nodes from hamsters infused with cells from nonimmune donors. Furthermore, recipients of immune T cells failed to develop antitreponemal antibodies 21 days after challenge. Enriched T cells were obtained by flow cytometric separation by using monoclonal anti-Ia antibody 14-4-4s, which identified hamster B cells. Flow cytometric analysis by two-color immunofluorescent staining with anti-hamster-immunoglobulin and monoclonal anti-Ia antibody 14-4-4s confirmed that monoclonal anti-Ia antibody 14-4-4s recognized B cells. In addition, lymph node cells obtained after treatment with anti-Ia monoclonal antibody 14-4-4s and complement were 97% T cells, as determined by monoclonal antibody 20, a hamster T-cell marker. These results demonstrated that highly enriched T cells (immunoglobulin negative, Ia negative) from T. pallidum subsp. pertenue-immune hamsters conferred partial protection on hamsters against infection with T. pallidum subsp. pertenue.
机译：细胞介导的免疫力对梅毒螺旋体亚种感染的作用。人类或实验动物的持久性尚不清楚。仓鼠皮下注射来自苍白锥虫亚种的4 x 10（6）普通淋巴结细胞或富集的淋巴结T细胞（免疫球蛋白阴性，Ia阴性）。百日咳免疫的仓鼠抵抗苍白锥虫亚种的攻击。持久性与注入非免疫供体细胞的仓鼠淋巴结相比，接受免疫细胞的仓鼠淋巴结的重量更轻，且截短的染色体短。此外，免疫T细胞的接受者在攻击后21天未能产生抗梯蛋白抗体。使用单克隆抗Ia抗体14-4-4s通过流式细胞术分离获得富集的T细胞，该抗体可鉴定仓鼠B细胞。用抗仓鼠免疫球蛋白和单克隆抗Ia抗体14-4-4s进行双色免疫荧光染色的流式细胞仪分析证实，单克隆抗Ia抗体14-4-4s识别B细胞。另外，用抗Ia单克隆抗体14-4-4s和补体处理后获得的淋巴结细胞是97％的T细胞，由仓鼠T细胞标志物单克隆抗体20确定。这些结果证明来自苍白锥虫亚种的高度富集的T细胞（免疫球蛋白阴性，Ia阴性）。永久免疫的仓鼠为仓鼠提供了部分保护，使其免受苍白锥虫亚种的感染。持久性

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号