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首页> 外文期刊>Infection and immunity >Specificity and Mechanism of Immunoglobulin M (IgM)- and IgG-Dependent Protective Immunity to Larval Strongyloides stercoralis in Mice
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Specificity and Mechanism of Immunoglobulin M (IgM)- and IgG-Dependent Protective Immunity to Larval Strongyloides stercoralis in Mice

机译：免疫球蛋白M（IgM）和IgG依赖的小鼠幼虫Strongyloides stercoralis的保护性免疫的特异性和机制

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Protective immunity in mice to the infective third-stage larvae (L3) of Strongyloides stercoralis was shown to be dependent on immunoglobulin M (IgM), complement activation, and granulocytes. The objectives of the present study were to determine whether IgG was also a protective antibody isotype and to define the specificity and the mechanism by which IgG functions. Purified IgG recovered from mice 3 weeks after a booster immunization with live L3 was shown to transfer high levels of protective immunity to na?ve mice. IgG transferred into mice treated to block complement activation or to eliminate granulocytes failed to kill the challenge larvae. Transfer of immune IgG into IL-5 knockout (KO) mice, which are deficient in eosinophils, resulted in larval attrition, while transfer into FcRγ KO mice did not result in larval killing. These findings suggest that IgG from mice immunized with live L3 requires complement activation and neutrophils for killing of L3 through an antibody-dependent cellular cytotoxicity (ADCC) mechanism. This is in contrast to the results of investigations using IgM from mice immunized with live L3 and IgG from mice immunized with larval antigens soluble in deoxycholate in which protective immunity was shown to be ADCC independent. Western blot analyses with immune IgM and IgG identified few antigens recognized by all protective antibody isotypes. Results from immunoelectron microscopy demonstrated that the protective antibodies bound to different regions in the L3. It was therefore concluded that while IgM and IgG antibodies are both protective against larval S. stercoralis, they recognize different antigens and utilize different killing mechanisms.
机译：小鼠对>类固醇类感染性第三阶段幼虫（L3）的保护性免疫依赖于免疫球蛋白M（IgM），补体激活和粒细胞。本研究的目的是确定IgG是否也是保护性抗体同种型，并确定IgG功能的特异性和机理。用活的L3加强免疫3周后，从小鼠中回收的纯化的IgG被证明可以将高水平的保护性免疫转移给幼稚的小鼠。将IgG转移至经处理以阻断补体激活或消除粒细胞的小鼠中，未能杀死攻击幼虫。免疫性IgG转移到缺乏嗜酸性粒细胞的IL-5敲除（KO）小鼠中会导致幼虫减员，而转移到FcRγKO小鼠中不会导致幼虫被杀死。这些发现表明，用活的L3免疫的小鼠的IgG需要补体激活和嗜中性粒细胞才能通过抗体依赖性细胞毒性（ADCC）机制杀死L3。这与使用活L3免疫的小鼠的IgM和用可溶于脱氧胆酸盐的幼虫抗原免疫的小鼠的IgG的研究结果相反，其中保护性免疫被证明是ADCC独立的。用免疫IgM和IgG进行的蛋白质印迹分析确定了少数被所有保护性抗体同种型识别的抗原。免疫电子显微镜的结果表明，保护性抗体与L3的不同区域结合。因此得出结论，尽管IgM和IgG抗体都对幼虫 S具有保护作用。甾体，它们识别不同的抗原并利用不同的杀伤机制。

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