...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Microarray-Based Identification of htrA, a Streptococcus pneumoniae Gene That Is Regulated by the CiaRH Two-Component System and Contributes to Nasopharyngeal Colonization
【24h】

Microarray-Based Identification of htrA, a Streptococcus pneumoniae Gene That Is Regulated by the CiaRH Two-Component System and Contributes to Nasopharyngeal Colonization

机译：基于微阵列的htrA鉴定，htrA是由CiaRH两组分系统调节并有助于鼻咽定植的肺炎链球菌基因

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nasopharyngeal carriage is the reservoir from which most disease with Streptococcus pneumoniae arises. Survival as a commensal in this environment is likely to require a set of adaptations distinct from those needed to cause disease, some of which may be mediated by two-component signal transduction systems (TCSTS). We examined the contributions of nine pneumococcal TCSTS to the process of nasopharyngeal colonization by using an infant rat model. Whereas deletions in all but one of these systems have been associated previously with a high degree of attenuation in a murine model of pneumonia, only the CiaRH system was necessary for efficient carriage. Transcriptional analysis by using microarray hybridization identified a locus consisting of two adjacent genes, htrA and spoJ, that was specifically and strongly downregulated in a ΔciaRH-null mutant. A S. pneumoniae strain lacking the htrA gene encoding a putative serine protease, but not one lacking spoJ, showed decreased fitness in a competitive model of colonization, a finding consistent with this gene mediating a portion of the carriage deficit observed with the ΔciaRH strain.
机译：鼻咽运输是大多数肺炎链球菌疾病的发源地。要在这种环境中生存，必须要有一套与引起疾病所需的适应不同的适应，其中一些适应可能由两组分信号转导系统（TCSTS）介导。我们使用婴儿大鼠模型研究了九种肺炎球菌TCSTS对鼻咽定植过程的贡献。尽管除这些系统之一外，所有这些系统中的缺失先前都与鼠型肺炎模型中的高度减毒有关，但只有CiaRH系统才能有效携带。使用微阵列杂交的转录分析确定了由两个相邻基因 htrA 和 spoJ 组成的基因座，这些基因在Δ ciaRH 中被特异地强烈下调。 -null突变体。一个 S。缺乏编码假定的丝氨酸蛋白酶的 htrA 基因的肺炎菌株，但没有缺乏 spoJ 的菌株，在竞争性定殖模型中显示适应性降低，这一发现与该基因介导了Δ ciaRH 菌株观察到的部分运输缺陷。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号