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首页> 外文期刊>Infection and immunity >Serum Opacity Factor (SOF) of Streptococcus pyogenes Evokes Antibodies That Opsonize Homologous and Heterologous SOF-Positive Serotypes of Group A Streptococci
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Serum Opacity Factor (SOF) of Streptococcus pyogenes Evokes Antibodies That Opsonize Homologous and Heterologous SOF-Positive Serotypes of Group A Streptococci

机译：化脓性链球菌的血清不透明因子（SOF）引起与A组链球菌的同源和异源SOF阳性血清型调理的抗体

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Serum opacity factor (SOF) is a protein expressed by Streptococcus pyogenes that opacifies mammalian serum. SOF is also a virulence factor of S. pyogenes, but it has not been previously shown to elicit a protective immune response. Herein, we report that SOF evokes bactericidal antibodies against S. pyogenes in humans, rabbits, and mice. Rabbit antiserum against purified recombinant SOF2 opsonized SOF-positive M type 2, 4, and 28 S. pyogenes in human blood but had no effect on SOF-negative M type 5 S. pyogenes. Furthermore, affinity-purified human antibodies against SOF2 also opsonized SOF-positive streptococci. A combination of antisera against M2 and SOF2 proteins was dramatically more effective in killing streptococci than either antiserum alone, indicating that antibodies against SOF2 enhance the opsonic efficiency of M protein antibodies. Mice tolerated an intravenous injection of 100 μg of SOF without overt signs of toxicity, and immunization with SOF protected mice against challenge infections with M type 2 S. pyogenes. These data indicate that SOF evokes opsonic antibodies that may protect against infections by SOF-positive serotypes of group A streptococci and suggest that different serotypes of SOF have common epitopes that may be useful vaccine candidates to protect against group A streptococcal infections.
机译：血清不透明因子（SOF）是化脓性链球菌（emem）表达的一种蛋白，可以使哺乳动物的血清不透明。 SOF还是 S的毒力因子。化脓，但以前尚未证明它能引起保护性免疫反应。在此，我们报道SOF引发针对 S的杀菌抗体。化脓菌在人，兔子和小鼠中发生。兔抗纯化的重组SOF2调理SOF阳性M型2、4和28 S的血清。化脓性在人血中，但对SOF阴性的5型M S没有影响。化脓。此外，针对SOF2的亲和纯化人抗体也调理SOF阳性链球菌。抗M2和SOF2蛋白的抗血清的组合比单独使用任何一种抗血清，在杀死链球菌方面更有效，这表明抗SOF2的抗体增强了M蛋白抗体的调理作用。小鼠耐受100μgSOF的静脉内注射而没有明显的毒性迹象，并且用SOF免疫保护了小鼠免受M型2emS的攻击性感染。化脓。这些数据表明，SOF唤起了可以保护A组链球菌SOF阳性血清型感染的调理抗体，并表明SOF的不同血清型具有共同的表位，可能是预防A组链球菌感染的有用候选疫苗。

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