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首页> 外文期刊>Infection and immunity >Identification of immunogenic and antibody-binding membrane proteins of pathogenic Trichomonas vaginalis.
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Identification of immunogenic and antibody-binding membrane proteins of pathogenic Trichomonas vaginalis.

机译：病原性阴道毛滴虫的免疫原性和抗体结合膜蛋白的鉴定。

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Characterization of immunogenic Trichomonas vaginalis membrane proteins was accomplished by using extrinsically and intrinsically labeled organisms and a highly sensitive and specific radioimmunoprecipitation procedure. Intact motile trichomonads were compared with detergent extracts as a source of antigen in radioimmunoprecipitation experiments. Approximately 20 proteins accessible to antibody were identified and ranged in molecular weight from 200,000 to 20,000. Localization on the parasite surface of the highly immunogenic membrane proteins was attempted by using, as the indicator system, formaldehyde-fixed protein A-bearing Staphylococcus aureus pretreated with the various antiserum reagents and incubated with live, motile parasites. Also, indirect immunofluorescence with fluorescein isothiocyanate--anti-rabbit immunoglobulin was also employed after incubation of organisms with either control serum or antiserum from immunized rabbits or after treatment of trichomonads with the immunoglobulin G fraction from each respective serum. No immunoglobulin G antibody appeared to be directed at the anterior trichomonal flagella or the posterior axostyle, whereas strong fluorescence was detected throughout the rest of the T. vaginalis surface. The biological significance of these data is discussed.
机译：免疫原性阴道毛滴虫膜蛋白的表征是通过使用外部和内部标记的生物以及高度敏感和特异的放射免疫沉淀程序完成的。在放射免疫沉淀实验中，将完整的运动滴虫与去污剂提取物作为抗原来源进行了比较。鉴定出约20种可与抗体接触的蛋白质，分子量范围为200,000至20,000。通过使用预先用各种抗血清试剂预处理并与活的，能运动的寄生虫一起孵育的甲醛固定蛋白A的金黄色葡萄球菌作为指示剂系统，尝试在高度免疫原性膜蛋白的寄生虫表面上定位。同样，在将生物与来自免疫兔子的对照血清或抗血清孵育后的生物体中，或在用来自各个血清的免疫球蛋白G级分处理滴虫后，还使用了异硫氰酸荧光素-抗兔免疫球蛋白进行间接免疫荧光。似乎没有免疫球蛋白G抗体直接针对毛滴虫前鞭毛或后轴突，而在整个阴道T.其余表面均检测到强荧光。讨论了这些数据的生物学意义。

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