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首页> 外文期刊>Infection and immunity >In vitro parasite-monocyte interactions in human leishmaniasis: effect of enzyme treatments on attachment.
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In vitro parasite-monocyte interactions in human leishmaniasis: effect of enzyme treatments on attachment.

机译：人利什曼病中的体外寄生虫-单核细胞相互作用：酶处理对附着的影响。

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摘要

Essential to the pathogenesis of leishmaniasis is the ability of Leishmania spp. to attach to mononuclear phagocyte surfaces before entering this host cell which they parasitize. We have investigated the attachment phase of infection in vitro by quantitating the percent of human peripheral blood monocytes pretreated with cytochalasin (to prevent parasite entry) to which tissue-derived L. tropica amastigotes will attach during coincubation at 37 degrees C in serum-free medium. We determined that pretreatment of parasites with trypsin, chymotrypsin, Pronase, and neuraminidase reduced attachment. In contrast, parasites treated with beta-galactosidase had an enhanced ability to attach to host cells. Treatment of monocytes with chymotrypsin and Pronase, but not with trypsin or neuraminidase, reduced attachment of untreated amastigotes. We propose that in vitro amastigote attachment under serum-free conditions depends on the interaction of protein determinants on the surface of both parasite and host cell.
机译：对利什曼病的发病机制至关重要的是利什曼原虫的能力。进入单核吞噬细胞表面，然后进入被寄生的宿主细胞。我们通过定量用细胞松弛素预处理（以防止寄生虫进入）的人外周血单核细胞的百分比来研究感染的附着阶段，在37°C无血清培养基中共孵育过程中，组织来源的嗜热乳杆菌将附着于组织。我们确定用胰蛋白酶，胰凝乳蛋白酶，链霉蛋白酶和神经氨酸酶预处理寄生虫可减少附着。相反，用β-半乳糖苷酶处理的寄生虫具有增强的附着于宿主细胞的能力。用胰凝乳蛋白酶和链霉蛋白酶治疗单核细胞，但不用胰蛋白酶或神经氨酸酶治疗单核细胞，减少了未经处理的变形虫的附着。我们建议在无血清条件下的体外鞭毛体附着取决于寄生虫和宿主细胞表面上蛋白质决定簇的相互作用。

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