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首页> 外文期刊>Infection and immunity >Genetic transformation of putative cariogenic properties in Streptococcus mutans.
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Genetic transformation of putative cariogenic properties in Streptococcus mutans.

机译：变形链球菌推定的致癌特性的遗传转化。

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摘要

Rough colonial morphology and bacteriocin production, two properties which may be associated with the cariogenicity of Streptococcus mutans, were transformed into several strain GS-5 mutants defective in each respective property. Transformation was determined by observing the frequency of cotransfer of these properties with different reference markers. The rough colonial transformants were identical to the parental GS-5 strain with respect to ability to synthesize water-insoluble glucans and undergo in vitro sucrose-dependent colonization of glass surfaces. Alterations in the growth medium and the concentration of the initial cell inoculum resulted in an approximate 10-fold increase in the frequency of transformation of strain GS-5 compared to previous observations.
机译：粗糙的殖民地形态和细菌素生产，可能与变形链球菌的致龋性相关的两个特性，被转化为几个在各自特性上有缺陷的GS-5菌株。通过观察这些特性与不同参考标记的共转移频率来确定转化。就合成水不溶性葡聚糖和进行蔗糖依赖性玻璃表面的体外定殖的能力而言，粗糙的结肠转化体与亲本GS-5菌株相同。与先前的观察结果相比，生长培养基的变化和初始细胞接种物的浓度导致菌株GS-5的转化频率增加了约10倍。

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