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首页> 外文期刊>Infection and immunity >Enzymatic release of germ tube-specific antigens from cell walls of Candida albicans.
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Enzymatic release of germ tube-specific antigens from cell walls of Candida albicans.

机译：从白色念珠菌的细胞壁中酶释放胚管特异性抗原。

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The differences between blastospores and germ tubes of Candida albicans, as previously shown by immunofluorescence, were further studied by comparing digests of cell walls of both growth forms. Organisms were surface labeled with 125I, and cell walls were digested enzymatically. When Zymolase digests were treated with polyclonal, polyspecific antiserum to C. albicans 441B, which stains only germ tubes in immunofluorescence assays, components of molecular weights 200,000 and 155,000 were immunoprecipitated from digests of germ tubes of strain B311, but nothing was recovered from blastospores. Whereas the 200,000-molecular-weight component was found in the three strains tested, the 155,000-molecular-weight component was found only in strain B311. When Zymolase digests were treated with unadsorbed antiserum, which stains both blastospores and germ tubes in immunofluorescence assays, an additional component was precipitated from digests of both growth forms with a molecular weight greater than the 200,000-molecular-weight marker. All three antigens were mannoproteins, as was shown by their abilities to bind concanavalin A and to be labeled by 125I. Also, all antigens were located on the cell surface, as was shown by the following criteria: adsorption of antisera with live organisms removed antibody to these components, and antibody eluted from surfaces of whole organisms precipitated all components. Components common to both growth forms, as well as germ tube-specific components, were detected in trypsin and chymotrypsin digests, but their molecular weights differed from those of Zymolase digests. Thus, germ tube-specific surface determinants as well as determinants common to both growth forms were detected on enzymatically released cell wall components.
机译：通过比较两种生长形式的细胞壁消化物，进一步研究了念珠菌和白色念珠菌的胚芽孢和胚管之间的差异。有机体表面用125 I标记，并用酶消化细胞壁。当用多克隆抗白色念珠菌441B的多特异性多特异性抗血清处理Zymolase消化物时，在免疫荧光测定中仅染色菌管，分子量200,000和155,000的成分从菌株B311的菌管消化物中被免疫沉淀，但是从芽孢杆菌中没有回收到任何东西。尽管在测试的三个菌株中发现了200,000分子量组分，但是仅在菌株B311中发现了155,000分子量组分。当用未吸附的抗血清对Zymolase消化液进行处理时，在免疫荧光测定中，该血清同时感染了芽孢和胚芽管，从两种生长形式的消化液中沉淀出了分子量大于200,000分子量标记的其他成分。所有三种抗原均为甘露糖蛋白，如其结合伴刀豆球蛋白A并由125I标记的能力所显示。同样，所有抗原都位于细胞表面，如以下标准所示：活生物体对抗血清的吸附去除了针对这些成分的抗体，而从整个生物体表面洗脱的抗体使所有成分沉淀。在胰蛋白酶和胰凝乳蛋白酶消化物中检测到两种生长形式共有的组分以及胚管特异性组分，但是它们的分子量不同于Zymolase消化的分子量。因此，在酶释放的细胞壁成分上检测到了生殖管特异性表面决定簇以及两种生长形式共有的决定簇。

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