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首页> 外文期刊>Infection and immunity >Antibodies that bind to fimbriae block adhesion of Streptococcus sanguis to saliva-coated hydroxyapatite.
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Antibodies that bind to fimbriae block adhesion of Streptococcus sanguis to saliva-coated hydroxyapatite.

机译：结合菌毛的抗体会阻止血链球菌粘附到唾液包覆的羟基磷灰石上。

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Antibodies raised against a fimbriated, adhesive strain of Streptococcus sanguis (FW213) were found to block the adhesion of this organism to saliva-coated hydroxyapatite. Antibodies were made specific for adhesion antigens by adsorption with isogenic, nonadhesive mutants (for rabbit polyclonal adsorbed antibody) or selection based on nonreactivity with two nonadhesive mutants (for monoclonal antibody). Rabbit antibody raised against isogenic, nonfimbriated nonadhesive mutants served as a control for antibodies present, but not related to fimbriation. Adsorbed antibody and monoclonal antibody were shown to be specific for fimbriae (antigen 1), since both antibodies could be seen by immune electron microscopy to bind 3.6-nm fimbriae, reacted only with the fimbriated parent and not the mutants in a whole bacterial cell enzyme-linked immunosorbent assay, and could immunoprecipitate fimbriae from fimbrial extracts of FW213. Antibodies isolated from preimmune and mutant sera did not react with fimbriae in any of the above assays. Only adsorbed antibody and monoclonal antibody were capable of blocking the adhesion of FW213 to saliva-coated hydroxyapatite. Adsorbed antibody, purified to immunoglobulin G (IgG), was an effective inhibitor of adhesion without causing interfering cellular aggregation. Monoclonal IgG, papain-cleaved to Fab fragments to prohibit cell-to-cell cross-linking, was also a potent inhibitor of S. sanguis FW213 adhesion. Both IgG from mutant sera and Fab fragments from normal mouse IgG could not be shown to block adhesion. These data further support the hypothesis that S. sanguis fimbriae are involved in adhesion.
机译：发现针对有纤毛的黏附性血链球菌（FW213）产生的抗体可阻止该生物粘附至唾液包被的羟基磷灰石。通过用等基因的非粘附性突变体（对于兔多克隆吸附抗体）吸附或基于与两个非粘附性突变体的非反应性选择（对于单克隆抗体），使抗体对粘附抗原具有特异性。针对同基因的，非成膜的非粘附性突变株产生的兔抗体可作为存在抗体的对照，但与成纤维无关。已显示吸附的抗体和单克隆抗体对菌毛具有特异性（抗原1），因为通过免疫电子显微镜可以看到这两种抗体均结合了3.6 nm菌毛，仅与菌毛亲本反应，而不与整个细菌细胞酶中的突变体反应联免疫吸附试验，可从FW213的纤维提取物中免疫沉淀菌毛。在上述任何检测中，从免疫前和突变血清中分离的抗体均未与菌毛反应。仅吸附的抗体和单克隆抗体能够阻断FW213对唾液包被的羟基磷灰石的粘附。纯化为免疫球蛋白G（IgG）的吸附抗体是一种有效的粘附抑制剂，不会引起干扰细胞的聚集。木瓜蛋白酶切割成Fab片段以阻止细胞间交联的单克隆IgG也是桑氏葡萄球菌FW213粘附的有效抑制剂。突变血清的IgG和正常小鼠IgG的Fab片段均未显示出阻断粘附的作用。这些数据进一步支持了假血链球菌菌丝参与粘附的假说。

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