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首页> 外文期刊>Infection and immunity >Biologically active antibodies elicited by a synthetic circumsporozoite peptide of Plasmodium knowlesi administered in saline with a muramyl dipeptide derivative.
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Biologically active antibodies elicited by a synthetic circumsporozoite peptide of Plasmodium knowlesi administered in saline with a muramyl dipeptide derivative.

机译：具有生理活性的抗体，是由在血浆中与鼠酰胺基二肽衍生物一起施用的诺氏疟原虫的合成环子孢子肽引发的。

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摘要

A synthetic peptide whose sequence was derived from the circumsporozoite protein of Plasmodium knowlesi coupled to bovine gamma globulin has been shown to be immunogenic when administered with Freund complete adjuvant. The present experiments were designed to test the immunogenicity of the peptide when attached to a tetanus toxoid carrier and administered with alum or murabutide, both acceptable clinical adjuvants. In both cases, the use of an adjuvant increased the levels of circulating anti-peptide antibodies over those observed when no adjuvant was used. However, when the antisera were tested for reactivity with the native protein, animals of the group receiving the conjugate associated with murabutide always had titers greatly exceeding those observed in animals that received the conjugate with alum. Moreover, the sera of the murabutide-treated group were shown to be more active in eliciting shedding of the circumsporozoite protein than were sera of animals of the Freund complete adjuvant-treated group. The use of tetanus toxoid in secondary immunizations could be eliminated when the mice primed with peptide-tetanus toxoid and murabutide were boosted with a polymer of the peptide. The results indicate that the synthetic malarial peptide-tetanus toxoid conjugate is capable of stimulating high levels of biologically active antibodies only when administered with murabutide.
机译：当与弗氏完全佐剂一起给药时，其序列衍生自与疟原虫丙种球蛋白偶联的诺氏疟原虫的环子孢子蛋白的合成肽已显示出免疫原性。设计本实验以测试当肽与破伤风类毒素载体连接并与明矾或murabutide一起施用时的免疫原性，这两种都是可接受的临床佐剂。在这两种情况下，佐剂的使用都比未使用佐剂时增加了循环抗肽抗体的水平。然而，当测试抗血清与天然蛋白的反应性时，接受与murabutide结合的缀合物的组的动物的滴度总是大大超过接受明矾的缀合物的动物中观察到的滴度。而且，与弗氏完全佐剂处理组的动物的血清相比，村丁肽处理组的血清显示出在引起环子孢子蛋白脱落方面更活跃。当用肽-破伤风类毒素和murabutide引发的小鼠用该肽的多聚体加强免疫时，可以消除破伤风类毒素在二次免疫中的使用。结果表明，仅当与murabutide一起施用时，合成的疟疾肽-破伤风类毒素缀合物才能够刺激高水平的生物活性抗体。

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