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首页> 外文期刊>Infection and immunity >Distinct bacteriocin groups correlate with different groups of Streptococcus mutans plasmids.
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Distinct bacteriocin groups correlate with different groups of Streptococcus mutans plasmids.

机译：不同的细菌素基团与变形链球菌质粒的不同组相关。

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A correlation between the presence of 5.6-kilobase plasmids and bacteriocin activity was found in human-derived strains of Streptococcus mutans. Compared with bacteriocin activity of randomly selected clinical isolates of plasmid-negative strains, bacteriocin activity of plasmid-positive strains significantly inhibited not only two laboratory strains of S. mutans, OMZ176 and AHT (P less than 0.0001 and P = 0.038, respectively), but most plasmid-negative clinical isolates as well (P = 0.0005). Comparisons of inhibition between pairs of plasmid-positive strains revealed two groups, group I and group II, that produced distinct bacteriocins we designated as mutacin I and mutacin II, respectively. Within each group, a strain produced inhibitory activity against all the members of the other group but against no members of its own group. Plasmid DNA from plasmid-positive strains of each group was isolated and analyzed by restriction endonuclease digestion. Plasmids from the two groups that were apparently identical in size differed in digestion patterns for EcoRI, HaeIII, and TaqI, even though six TaqI fragments appeared to be common to all. Based on bacteriocin profiles and restriction enzyme digests, two distinct groups of plasmid-positive S. mutans strains emerged. Although the bacteriocin activity of plasmid-positive strain LM7 (serotype e) placed it in group I, clear differences in restriction digests distinguished it from the other plasmid-containing strains.
机译：在人源性变形链球菌菌株中发现了5.6碱基碱基的质粒与细菌素活性之间的相关性。与随机选择的质粒阴性菌株临床分离株的细菌素活性相比，质粒阳性菌株的细菌素活性不仅显着抑制了变形链球菌的两个实验室菌株OMZ176和AHT（分别小于0.0001和P = 0.038，但大多数质粒阴性临床分离株也是如此（P = 0.0005）。对质粒阳性菌株对之间抑制作用的比较揭示了两组，分别为I组和II组，它们分别产生了不同的细菌素，我们分别将其命名为mutacin I和mutacin II。在每一组中，菌株对另一组的所有成员产生抑制活性，但对本组的任何成员均没有抑制活性。分离每组质粒阳性菌株的质粒DNA，并通过限制性核酸内切酶消化进行分析。两组大小相同的质粒的消化模式在EcoRI，HaeIII和TaqI的消化模式上有所不同，尽管六个TaqI片段似乎是所有人都共有的。基于细菌素谱和限制酶消化，出现了两组不同的质粒阳性变形链球菌菌株。尽管质粒阳性菌株LM7（血清型e）的细菌素活性处于I组，但限制性酶切消化的明显差异将其与其他含质粒菌株区分开来。

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