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首页> 外文期刊>Infection and immunity >Some galE mutants of Salmonella choleraesuis retain virulence.
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Some galE mutants of Salmonella choleraesuis retain virulence.

机译：霍乱沙门氏菌的一些galE突变体保留有毒力。

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galE mutants were isolated from three mouse-virulent strains of Salmonella choleraesuis (of group C1, O antigen 6,7) by selection for resistance to 2-deoxygalactose. The galE derivative of strain 381 comprised two components: galactose sensitive, thought to be the original mutant; and galactose resistant, presumably by a second mutation reducing galK or galT function or both. The galactose-sensitive component had an intraperitoneal 50% lethal dose for BALB/c mice of ca. 4 X 10(6) CFU, whereas the galactose-resistant component was about as virulent as its gal+ parent, with a 50% lethal dose of ca. 100 CFU. The galE mutant of strain 110 was somewhat sensitive to galactose, as shown by retardation of growth; its 50% lethal dose, ca. 500 CFU, was not much greater than the ca. 200 CFU value for its parent. The galE mutant of strain 117 showed the same partial sensitivity to galactose as strain 110 galE, but was nonvirulent (50% lethal dose of ca. 10(6) CFU versus ca. 400 CFU for its parent). Growth on galactose-supplemented medium restored the smooth phenotype, as indicated by phage sensitivity to three of the four galE strains, but only partially so for the strain 117 galE mutant. The retention of parental virulence by galE mutants of S. choleraesuis which are galactose resistant or somewhat galactose sensitive contrasts with the greatly reduced virulence of galactose-resistant galE mutants of Salmonella typhimurium and Salmonella typhi; this difference may result from the absence of galactose from the O repeat unit in the lipopolysaccharide of group C1 salmonellae.
机译：通过选择对2-脱氧半乳糖的抗性，从三株猪霍乱沙门氏菌（C1组，O抗原6,7）的毒力强毒株中分离出galE突变体。菌株381的galE衍生物包含两个成分：对半乳糖敏感，被认为是原始突变体;对半乳糖敏感。对半乳糖具有抗性，大概是由于第二次突变导致galK或galT功能或两者兼而有之。半乳糖敏感成分对BALB / c小鼠的腹膜内致死剂量约为50％。 4 X 10（6）CFU，而耐半乳糖成分的毒性与其gal +母体一样强，大约50％的致死剂量。 100 CFU。菌株110的galE突变体对半乳糖有些敏感，表现为生长迟缓。其50％致死剂量，约。 500 CFU，不大于ca。其父级的200 CFU值。菌株117的galE突变体对半乳糖的部分敏感性与菌株110 galE相同，但无毒（50％致死剂量的约10（6）CFU与其亲本的约400 CFU）。如对四个galE菌株中的三个菌株的噬菌体敏感性所表明的，在补充有半乳糖的培养基上的生长恢复了平滑的表型，但是对于菌株117 galE突变体仅部分地如此。耐半乳糖或有点对半乳糖敏感的霍乱链球菌galE突变体保留亲本毒力，而鼠伤寒沙门氏菌和鼠伤寒沙门氏菌的半乳糖抗性galE突变体的毒力大大降低。这种差异可能是由于C1沙门氏菌的脂多糖中O重复单元中的半乳糖不存在所致。

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