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首页> 外文期刊>Infection and immunity >Production, characterization, and antibody specificity of a mouse monoclonal antibody reactive with Cryptococcus neoformans capsular polysaccharide.
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Production, characterization, and antibody specificity of a mouse monoclonal antibody reactive with Cryptococcus neoformans capsular polysaccharide.

机译：与新型隐球菌荚膜多糖反应的小鼠单克隆抗体的产生，表征和抗体特异性。

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Two monoclonal immunoglobulin G1 antibodies reacting with Cryptococcus neoformans capsular polysaccharide (CNPS) were produced in mice by using a carefully defined procedure for immunization with unmodified CNPS purified from C. neoformans serotype A. Since the antibodies were found to have the same pattern of specificity, only one of them (E1) is described. This anti-CNPS monoclonal antibody reacted with the glucuronoxylomannan component of CNPS but not with the constituent monosaccharides or with the mannose alpha(1----3)-linked oligosaccharide structures present on CNPS. E1 appeared to be specific for C. neoformans serotype A by agglutination of whole cells; it was specific for soluble CNPS A by gel immunoprecipitation. However, indirect immunofluorescence and competitive-binding enzyme-linked immunosorbent assay experiments showed low levels of cross-reactivity with serotypes B and D but not with serotype C. Concentrations 10,000 times higher for serotypes B and D cells than for serotype A cells were required for a 50% inhibition of E1 anti-CNPS A activity as measured by enzyme-linked immunosorbent assay. Among the other yeasts tested, a cross-reaction was only detected with Trichosporon beigelii. The four serotypes of C. neoformans could be distinguished based on intensities and patterns of fluorescence in an indirect immunofluorescence assay using the monoclonal anti-CNPS A antibody. Monoclonal anti-CNPS A antibodies could be useful for fundamental studies on the glucuronoxylomannan structure, as well as for clinical applications such as serotyping and possibly the serological diagnosis of cryptococcosis.
机译：使用精心定义的程序，用从新孢子虫血清型A纯化的未经修饰的CNPS进行免疫接种，在小鼠中产生了两种与新隐球菌荚膜多糖（CNPS）反应的单克隆免疫球蛋白G1抗体。由于发现这些抗体具有相同的特异性模式，仅描述了其中一个（E1）。该抗CNPS单克隆抗体与CNPS的葡糖醛酸氧甘露聚糖成分反应，但不与CNPS上存在的单糖组成成分或与甘露糖α（1-3）连接的寡糖结构反应。通过全细胞凝集，E1似乎对新孢子虫血清型A具有特异性。通过凝胶免疫沉淀对可溶性CNPS A具有特异性。但是，间接免疫荧光和竞争性结合酶联免疫吸附试验表明，与血清型B和D的交叉反应水平较低，但与血清型C的交叉反应水平较低。对于血清型B和D细胞，其浓度要比血清型A细胞高10,000倍。通过酶联免疫吸附法测定，对E1抗CNPS A活性的抑制率为50％。在测试的其他酵母中，仅与Trichosporon米色酵母菌检测到交叉反应。在使用单克隆抗CNPS A抗体的间接免疫荧光测定中，可以基于荧光的强度和模式来区分四种新孢子虫血清型。单克隆抗CNPS A抗体可能对葡糖醛酸甘露聚糖结构的基础研究以及临床应用（如血清分型和可能的隐球菌血清学诊断）有用。

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