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首页> 外文期刊>Infection and immunity >Mutants of Actinomyces viscosus T14V lacking type 1, type 2, or both types of fimbriae.
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Mutants of Actinomyces viscosus T14V lacking type 1, type 2, or both types of fimbriae.

机译：缺乏1型，2型或两种类型的菌毛的放线菌粘性T14V突变体。

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Mutants of Actinomyces viscosus T14V lacking type 1 or type 2 fimbriae or both were selected by their failure to react with rabbit antibodies against either or both fimbrial antigens. Immunospecific double labeling with iron dextran and ferritin-conjugated antibodies showed two types of fimbriae on individual cells of the parent organism, a single type on mutant strains with type 1+2- and type 1-2+ fimbriae and no labeled or unlabeled fimbriae on a type 1-2- fimbria-deficient strain. The mutational loss of one fimbrial antigen did not appear to affect expression of the other, since bacteria with one or two types of fimbriae bound similar amounts of a monoclonal antibody directed against the fimbrial antigen present on both bacterial phenotypes. The strong adsorption of strains with type 1+2+ or 1+2- fimbriae to saliva-treated hydroxyapatite and weak adsorption of those with type 1-2+ or no fimbriae was consistent with the known involvement of type 1 fimbriae in this attachment process. Similarly, the A. viscosus lectin was clearly associated with the expression of type 2 fimbriae, since only the strains with type 1+2+ or 1-2+ fimbriae participated in lactose-sensitive coaggregations with Streptococcus sanguis 34. Further studies using the fimbria-deficient mutant strains showed that aggregation of A. viscosus T14V in the presence of sialidase-treated human saliva involved both types of fimbriae, whereas neither type was required for the lactose-resistant coaggregation of the organism with certain streptococcal strains.
机译：缺乏1型或2型菌毛或两者的粘性放线菌T14V突变体的选择是因为它们无法与针对任一或两种纤维抗原的兔抗体反应。用右旋糖酐铁和铁蛋白结合的抗体进行的免疫特异性双重标记在亲代生物的单个细胞上显示两种类型的菌毛，在具有1 + 2-型和1-2 +型菌毛的突变菌株上单种菌毛，而在其上没有标记或未标记的菌毛。 1-2型菌毛缺乏型菌株。一种纤维抗原的突变丢失似乎不会影响另一种抗原的表达，因为具有一种或两种类型菌毛的细菌会结合相似量的针对两种细菌表型上存在的纤维抗原的单克隆抗体。具有1 + 2 +或1 + 2-型菌毛的菌株对唾液处理的羟基磷灰石的强烈吸附以及具有1-2 +或无菌毛的菌株的弱吸附与已知的1型菌毛在该附着过程中的参与一致。同样，粘性黏凝素凝集素显然与2型菌毛的表达有关，因为只有1 + 2 +或1-2 +菌毛的菌株才与血链球菌34一起参与乳糖敏感的聚集。使用该菌毛的进一步研究缺陷型突变菌株显示，在唾液酸酶处理的人唾液存在下，粘稠曲霉T14V的聚集涉及两种类型的菌毛，而对于某些链球菌菌株而言，乳糖耐性性聚集不需要这两种类型。

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