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首页> 外文期刊>Infection and immunity >DNA probes specific for Entamoeba histolytica possessing pathogenic and nonpathogenic zymodemes.
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DNA probes specific for Entamoeba histolytica possessing pathogenic and nonpathogenic zymodemes.

机译：特异于溶组织变形杆菌的DNA探针，具有致病性和非致病性的酶模式。

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摘要

A number of DNA probes which hybridize to highly abundant DNA sequences of Entamoeba histolytica were developed. Variations in the hybridization patterns of different E. histolytica strains were detected with selected probes. Four types of restriction fragment length patterns were obtained. Of these, the first class belonged to E. invadens and E. histolytica-like var. Laredo. The next two classes consisted of various strains of E. histolytica which were originally isolated from symptomatic patients and possessed pathogenic patterns of isoenzymes (zymodemes), whereas the fourth group contained E. histolytica strains with nonpathogenic zymodemes obtained from asymptomatic carriers. DNA probes, based on DNA sequences specific to E. histolytica isolates with pathogenic and nonpathogenic zymodemes were isolated, and their nucleotide sequences were determined. These probes (P145 and B133) hybridized selectively to DNA of isolates possessing either pathogenic or nonpathogenic isoenzyme patterns. The newly developed probes could be useful for diagnostic purposes and could serve as tools to investigate the molecular basis of pathogenicity and the genetic mechanisms which regulate the variable aggressive behavior of the parasite.
机译：已开发出许多与高溶组织变形杆菌的DNA序列杂交的DNA探针。用选定的探针检测到不同的组织溶大肠杆菌菌株的杂交模式的变化。获得了四种类型的限制性片段长度模式。其中，第一类属于入侵大肠杆菌和类溶组织大肠杆菌。拉雷多接下来的两类包括最初从有症状患者中分离出来的各种溶血性大肠杆菌，并具有同工酶（zymodemes）的致病性模式，而第四类则是溶酶性大肠杆菌，其菌株是从无症状携带者获得的非致病性酶。基于具有致病性和非致病性zymodes的溶血性大肠杆菌分离株特异的DNA序列，分离DNA探针，并确定其核苷酸序列。这些探针（P145和B133）与具有致病性或非致病性同工酶模式的分离株的DNA选择性杂交。新开发的探针可用于诊断目的，并可作为研究致病性的分子基础和调节寄生虫可变侵袭行为的遗传机制的工具。

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