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首页> 外文期刊>Infection and immunity >Transcriptional mapping and nucleotide sequence of the Listeria monocytogenes hlyA region reveal structural features that may be involved in regulation.
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Transcriptional mapping and nucleotide sequence of the Listeria monocytogenes hlyA region reveal structural features that may be involved in regulation.

机译：单核细胞增生李斯特菌hlyA区的转录定位和核苷酸序列揭示了可能参与调控的结构特征。

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摘要

DNA sequence analysis of the regions adjacent to the hlyA gene, which encodes listeriolysin O, an essential virulence factor of Listeria monocytogenes, revealed the presence of two open reading frames (ORFs): ORF D located 304 base pairs downstream from hlyA, and ORF U located 224 base pairs upstream from and in opposite direction to hlyA. Promoter mapping performed with RNAs extracted from cells growing exponentially in rich medium showed that the three ORFs are independently transcribed. hlyA is transcribed from two promoters separated by 10 base pairs (P1 hlyA and P2 hlyA). ORF U is transcribed in the opposite direction from an adjacent promoter. These two promoter regions are separated by a palindromic sequence T-T-A-A-C-A-A/T-T-G-T-T-A-A. This palindrome was also found upstream from the ORF D promoter, suggesting that all three genes are similarly regulated.
机译：编码李斯特菌单核细胞增生李斯特氏菌必不可少的利斯特氏菌溶血素O的hlyA基因附近区域的DNA序列分析显示存在两个开放阅读框（ORF）：ORF D位于hlyA下游的304个碱基对，而ORF U位于hlyA上游且与hlyA相反的方向的224个碱基对。用从在丰富培养基中成倍增长的细胞中提取的RNA进行的启动子作图显示，这三个ORF是独立转录的。 hlyA从由10个碱基对分隔的两个启动子（P1 hlyA和P2 hlyA）转录。 ORF U与相邻启动子的转录方向相反。这两个启动子区域由回文序列T-T-A-A-C-A-A / T-T-G-T-T-A-A隔开。该回文在ORF D启动子的上游也被发现，表明所有三个基因都受到类似的调控。

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