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首页> 外文期刊>Infection and immunity >Primary structure of the porin protein of Haemophilus influenzae type b determined by nucleotide sequence analysis.
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Primary structure of the porin protein of Haemophilus influenzae type b determined by nucleotide sequence analysis.

机译：通过核苷酸序列分析确定b型流感嗜血杆菌的孔蛋白的一级结构。

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摘要

Sequencing techniques for single- and double-stranded DNA were used to determine the nucleotide sequence of the gene encoding P2, the major outer membrane (porin) protein of Haemophilus influenzae type b (Hib). The open reading frame encoding the P2 protein comprised 361 amino acid codons. Comparison of the inferred amino acid sequence with data obtained by amino acid sequencing of the N terminus of the mature or fully processed P2 protein revealed that this protein has a signal peptide composed of 20 amino acids. N-terminal amino acid sequencing of tryptic peptides derived from purified P2 allowed direct identification of 158 of the 341 amino acids in the fully processed P2 protein; there was 100% correlation between these amino acid sequences and that inferred from the nucleotide sequence. The amino acid sequence of Hib P2 protein had 23 to 25% homology with the sequence of the OmpF porin of Escherichia coli and with that of the Neisseria gonorrhoeae porin P.IA. Codon usage in the Hib P2 gene was significantly different from that observed for a gene encoding a porin of E. coli. DNA hybridization studies indicated that there is a single copy of the P2 gene in the Hib chromosome. The availability of the nucleotide and amino acid sequences for the Hib P2 protein will facilitate investigation of the antigenic characteristics and structure-function relationship of this porin.
机译：单链和双链DNA的测序技术用于确定编码P2基因的核苷酸序列，P2是b型流感嗜血杆菌（Hib）的主要外膜蛋白。编码P2蛋白的开放阅读框包含361个氨基酸密码子。将推断的氨基酸序列与通过对成熟或完全加工的P2蛋白的N末端进行氨基酸测序得到的数据进行比较，发现该蛋白具有由20个氨基酸组成的信号肽。从纯化的P2衍生的胰蛋白酶肽的N端氨基酸测序可直接鉴定完全加工的P2蛋白中341个氨基酸中的158个;这些氨基酸序列与从核苷酸序列推断出的序列之间存在100％的相关性。 Hib P2蛋白的氨基酸序列与大肠杆菌的OmpF孔蛋白和淋病奈瑟氏球菌的P.IA蛋白具有23-25％的同源性。 Hib P2基因的密码子使用与编码大肠杆菌孔蛋白的基因的密码子使用显着不同。 DNA杂交研究表明，Hib染色体中只有一个P2基因拷贝。 Hib P2蛋白的核苷酸和氨基酸序列的可用性将有助于研究该孔蛋白的抗原特性和结构-功能关系。

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