...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Isolation, structure, and immunogenicity of Pseudomonas aeruginosa immunotype 4 high-molecular-weight polysaccharide.
【24h】

Isolation, structure, and immunogenicity of Pseudomonas aeruginosa immunotype 4 high-molecular-weight polysaccharide.

机译：铜绿假单胞菌免疫型4高分子量多糖的分离，结构和免疫原性。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A high-molecular-weight, immunogenic form of the lipopolysaccharide O side chain of Pseudomonas aeruginosa Fisher immunotype 4 (type 4, International Antigenic Typing System 1, Lanyi O:6) was isolated and characterized. Analysis by nuclear magnetic resonance spectroscopy confirmed the structural similarity of this high-molecular-weight polysaccharide and the type 4 O side chain. The polysaccharide was immunogenic in rabbits and mice, eliciting opsonophagocytic killing antibodies. Immunization with the polysaccharide produced significant protection against homologous challenge in both burned and granulocytopenic mice. Naturally acquired opsonic killing antibodies to type 4 polysaccharide were present in sera from unimmunized normal adults at levels comparable to postimmunization levels achieved after immunization with other type-specific polysaccharides. The specificity of the naturally occurring antibodies for the O side chain was documented by immunoblot analysis and inhibition studies. Naturally occurring polysaccharide-specific antibodies were comparable in their protective activity against live challenge in neutropenic animals to immunization-induced murine antibodies with similar specificity. These data suggest that naturally occurring serum antibody to P. aeruginosa type 4 lipopolysaccharide O side chains in most adults is not distinguishable in quantity or quality from immunization-induced antibodies in mice; evaluation of type 4-specific vaccines in humans may be complicated by this finding.
机译：分离并鉴定了铜绿假单胞菌Fisher免疫类型4（类型4，国际抗原分型系统1，Lanyi O：6）的高分子量，免疫原性形式的脂多糖O侧链。通过核磁共振波谱法的分析证实了该高分子量多糖和4 O型侧链的结构相似性。多糖在兔和小鼠中具有免疫原性，引发调理吞噬杀伤抗体。多糖免疫在烧伤和粒细胞减少小鼠中产生了针对同源攻击的显着保护作用。来自未免疫正常成年人的血清中天然获得的针对4型多糖的调理性杀死抗体的水平与使用其他类型特异性多糖免疫后达到的免疫后水平相当。通过免疫印迹分析和抑制研究证明了天然存在的抗体对O侧链的特异性。天然存在的多糖特异性抗体在针对嗜中性白血球缺乏症动物的活体攻击方面的保护活性与免疫诱导的鼠特异性抗体相似。这些数据表明，在大多数成年人中，天然存在的针对铜绿假单胞菌4型脂多糖O侧链的血清抗体在数量或质量上与小鼠的免疫诱导抗体没有区别。这一发现可能会使对人类4型特异性疫苗的评估变得复杂。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号