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首页> 外文期刊>Infection and immunity >Two-step processing for activation of the cytolysin/hemolysin of Vibrio cholerae O1 biotype El Tor: nucleotide sequence of the structural gene (hlyA) and characterization of the processed products.
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Two-step processing for activation of the cytolysin/hemolysin of Vibrio cholerae O1 biotype El Tor: nucleotide sequence of the structural gene (hlyA) and characterization of the processed products.

机译：霍乱弧菌O1生物型El Tor的溶血素/溶血素激活过程分为两步：结构基因（hlyA）的核苷酸序列和加工产物的特征。

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摘要

Vibrio cholerae O1 biotype El Tor produces and secretes a 65-kDa cytolysin/hemolysin into the culture medium. We cloned the structural gene (hlyA) for the cytolysin from the total DNA of a V. cholerae O1 El Tor strain, N86. Nucleotide sequence analysis of hlyA revealed an open reading frame consisting of 2,223 bp which can code for a protein of 741 amino acids with a molecular weight of 81,961. Consistent with this, a 79-kDa protein was identified as the product of hlyA by maxicell analysis in Escherichia coli. N-terminal amino acids of this 79-kDa HlyA protein and those of a 65-kDa El Tor cytolysin purified from V. cholerae were Asn-26 and Asn-158, respectively. The 82- and 79-kDa precursors of the 65-kDa mature cytolysin were found in V. cholerae by pulse-chase labeling and Western blot (immunoblot) analysis of hlyA products. Hemolytic activity of the 79-kDa HlyA protein from E. coli was less than 5% that for the 65-kDa cytolysin from V. cholerae. Our results suggest that in V. cholerae, the 82-kDa preprotoxin synthesized in the cytoplasm is secreted through the membranes into the culture medium as the 79-kDa inactive protoxin after cleavage of the signal peptide and is then further processed into the 65-kDa active cytolysin by release of the N-terminal 15-kDa fragment.
机译：霍乱弧菌O1生物型El Tor可产生65 kDa溶血素/溶血素并将其分泌到培养基中。我们从霍乱弧菌O1 El Tor菌株N86的总DNA中克隆了细胞溶素的结构基因（hlyA）。 hlyA的核苷酸序列分析揭示了一个开放阅读框，包含2223 bp，可编码741个氨基酸的蛋白质，分子量为81,961。与此相一致，在大肠杆菌中通过maxicell分析鉴定出79kDa的蛋白质为hlyA的产物。从霍乱弧菌纯化的该79-kDa HlyA蛋白的N-末端氨基酸和65-kDa El Tor细胞溶素的N-末端氨基酸分别为Asn-26和Asn-158。通过脉冲追踪标记和hlyA产物的Western印迹（免疫印迹）分析，在霍乱弧菌中发现了65 kDa成熟溶素的82 kDa和79 kDa前体。来自大肠杆菌的79 kDa HlyA蛋白的溶血活性小于来自霍乱弧菌的65 kDa溶血素的溶血活性的5％。我们的结果表明，在霍乱弧菌中，在细胞质中合成的82 kDa的前毒素被信号肽切割后通过膜分泌为79 kDa的无活性毒素，然后被进一步加工成65 kDa的毒素。通过释放N端15-kDa片段来激活细胞溶素。

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