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首页> 外文期刊>Infection and immunity >Attaching effacement of the rabbit enterocyte brush border is encoded on a single 96.5-kilobase-pair plasmid in an enteropathogenic Escherichia coli O111 strain.
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Attaching effacement of the rabbit enterocyte brush border is encoded on a single 96.5-kilobase-pair plasmid in an enteropathogenic Escherichia coli O111 strain.

机译：在肠致病性大肠杆菌O111菌株中的单个96.5碱基对质粒上编码兔肠上皮细胞刷状缘的附着表面。

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摘要

An enteropathogenic Escherichia coli (EPE) O111 serotype a,b,H- strain carried the following four plasmids: pLV501 (96.5 kilobase pairs [kbp]) specifying resistance to chloramphenicol, tetracycline, and kanamycin; pLV502 (8 kbp) specifying ampicillin resistance; pLV503 (1.9 kbp) specifying streptomycin resistance; and pLV504 (80 kbp) with no resistance markers. This EPEC attached to HEp-2 cells to produce localized clumps of bacteria (localized adhesion) and attached intimately to the enterocyte surface, leading to loss of the brush border (attaching effacement). Plasmid pLV501 was also found to specify the ability to produce localized adhesion on HEp-2 cells and attaching effacement in a rabbit ileal explant model system. Restriction maps showed considerable dissimilarities between pLV501 and pMAR-2, an EPEC plasmid carrying the EPEC adherence factor (EAF) genes. Furthermore, pLV501 did not hybridize with the EAF probe, whereas pLV504 did. There was sequence homology between pLV501 and large plasmids in all seven other well-characterized EPEC, only five of which hybridized with the EAF probe. These findings indicate that pLV501 carries at least one of the genes responsible for production of the brush border damage characteristic of EPEC.
机译：肠致病性大肠杆菌（EPE）O111血清型a，b，H菌株携带以下四个质粒：pLV501（96.5碱基对[kbp]），指定对氯霉素，四环素和卡那霉素的抗性; pLV502（8 kbp），指定氨苄青霉素耐药性; pLV503（1.9 kbp），具有链霉素抗性;和没有抗性标记的pLV504（80 kbp）。该EPEC附着在HEp-2细胞上，产生局部的细菌团块（局部粘附），并紧密附着在肠上皮细胞表面，导致刷缘丢失（附着污损）。还发现质粒pLV501具有在兔回肠外植体模型系统中在HEp-2细胞上产生局部粘附和附着污损的能力。限制性酶切图显示pLV501和pMAR-2（携带EPEC粘附因子（EAF）基因的EPEC质粒）之间存在相当大的差异。此外，pLV501不与EAF探针杂交，而pLV504与EAF探针杂交。在所有其他七个充分表征的EPEC中，pLV501与大质粒之间存在序列同源性，其中只有五个与EAF探针杂交。这些发现表明pLV501携带至少一种负责产生EPEC的刷缘损伤特征的基因。

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