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首页> 外文期刊>Infection and immunity >Identification of larval cross-reactive and egg-specific antigens involved in granuloma formation in murine schistosomiasis mansoni.
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Identification of larval cross-reactive and egg-specific antigens involved in granuloma formation in murine schistosomiasis mansoni.

机译：鉴定曼氏血吸虫病鼠肉芽肿形成中涉及的幼虫交叉反应性和卵特异性抗原。

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Cross-reactive humoral immune responses between antigens of different developmental stages of the worm Schistosoma mansoni have previously been demonstrated. In contrast, information on antigenic cross-reactivity at the T-cell level is still very sparse. The present study examined the cross-reactive T-cell responses to eggs and crude and fractionated soluble egg antigens (SEA) in infected mice prior to (from 0 to 4 weeks of infection) and after (5 weeks and onwards) egg deposition. Splenic lymphocyte proliferation to unfractionated SEA was detected as early as 2 weeks postinfection and increased rapidly by 4 weeks postinfection. Injections of live eggs into the lungs of infected mice at 4 weeks postinfection demonstrated enhanced granuloma formation, indicating the presence of primed T cells that respond to egg antigens. Further experiments with the artificial granuloma model and polyacrylamide gel electrophoresis-separated SEA fractions demonstrated that in mice infected for 4 weeks the 60- to 66-, 93- to 125-, and greater than 200-kDa SEA fraction-coated beads elicited significant pulmonary granulomas. By 6 weeks postinfection, when eggs are deposited in the livers, in addition to the cross-reactive fractions (60 to 66, 93 to 125, and greater than 200 kDa), beads coated with fractions of 25 to 30, 32 to 38, and 70 to 90 kDa also elicited significant granulomatous reactions. These antigenic fractions are considered to have elicited egg stage-specific T-cell responsiveness. In addition hepatic granuloma T cells from the 6th week of infection demonstrated the strongest blastogenic response to the 60- to 66-kDa cross-reactive fraction. Thus, in vitro and in vivo experiments demonstrated T-cell cross-reactivity between the larval and egg stages of the worm. On the basis of these observations, the appearance of the primary circumovum granulomatous response in infected mice is considered to represent the sum of larval cross-reactive and egg-specific T-cell responsiveness.
机译：蠕虫曼氏血吸虫不同发育阶段的抗原之间的交叉反应性体液免疫反应已得到证实。相反，有关T细胞水平的抗原交叉反应性的信息仍然很少。本研究检查了感染前（感染0到4周）和沉积后（5周及以后）对受感染小鼠的卵以及粗制和分级可溶性蛋抗原（SEA）的交叉反应性T细胞反应。感染后2周即可检测到脾淋巴细胞向未分级SEA的增殖，感染后4周迅速增加。感染后4周，将活卵注射入感染小鼠的肺部显示出肉芽肿形成增强，表明存在对卵抗原有反应的引发T细胞的存在。用人工肉芽肿模型和聚丙烯酰胺凝胶电泳分离的SEA馏分进行的进一步实验表明，在感染了4周的小鼠中，60-66、66-，93-125-以及大于200kDa的SEA馏分包被的珠引发了明显的肺肉芽肿。感染后6周，当卵沉积在肝脏中时，除了交叉反应级分（60至66、93至125和大于200 kDa）外，珠子上还覆盖了25至30、32至38， 70至90 kDa的颗粒也引起明显的肉芽肿反应。这些抗原级分被认为引起了卵期特异性T细胞应答。另外，感染第6周的肝肉芽肿T细胞表现出对60-66kDa交叉反应部分最强的成胚反应。因此，体外和体内实验证明了蠕虫的幼虫和卵期之间的T细胞交叉反应。根据这些观察结果，被感染小鼠的原发性环周肉芽肿反应被认为代表了幼虫交叉反应性和卵特异性T细胞反应性的总和。

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