...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Relative availability of transferrin-bound iron and cell-derived iron to aerobactin-producing and enterochelin-producing strains of Escherichia coli and to other microorganisms.
【24h】

Relative availability of transferrin-bound iron and cell-derived iron to aerobactin-producing and enterochelin-producing strains of Escherichia coli and to other microorganisms.

机译：转铁蛋白结合的铁和细胞来源的铁对产气杆菌素和肠螯合素的大肠杆菌以及其他微生物的相对可用性。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A method is described for determination of the relative availability of transferrin-bound iron and cell-derived iron to microbial iron-scavenging mechanisms. This involved incubation of parallel cultures of microorganisms in dialysis tubes placed in RPMI 1640 tissue culture medium containing 30%-iron-saturated transferrin and K562 erythroleukemia cells. In one culture the transferrin was labelled with 59Fe and in the other the cells were labelled, and the relative uptake of radioiron by the microorganisms determined. The results showed that Staphylococcus epidermidis and Staphylococcus aureus acquired iron predominantly from cells, while Candida albicans and the enteropathogenic Escherichia coli NCTC 8623 tended to acquire iron from transferrin. E. coli K-12 strains W3110 and LG1705, which (like NCTC 8623) produce the siderophore enterochelin but not aerobactin, acquired predominantly transferrin-bound iron, whereas the related E. coli strains LG1315 and LG1628, which produce aerobactin but not enterochelin, showed a preference for cell-derived iron. When the cells were incubated in the presence of 59Fe-labelled transferrin and 55Fe-labelled ferritin, no difference in relative availability of iron to E. coli was observed, suggesting that differences in the ability of aerobactin and enterochelin to remove iron from intracellular ferritin were not responsible for this preference. These results may help to explain why production of aerobactin, despite its relatively low affinity for iron, is more closely associated with invasiveness in E. coli than is enterochelin production. Reduced availability of cell-bound iron during inflammation may contribute to antimicrobial defenses.
机译：描述了一种方法，用于确定运铁蛋白结合的铁和细胞衍生的铁对微生物清除铁的相对有效性。这涉及在置于含有30％铁饱和转铁蛋白和K562红白血病细胞的RPMI 1640组织培养基中的透析管中平行培养微生物。在一种培养物中，转铁蛋白用59Fe标记，在另一种中，细胞被标记，并确定了微生物对放射性铁的相对摄取。结果表明，表皮葡萄球菌和金黄色葡萄球菌主要从细胞中获取铁，而白色念珠菌和肠致病性大肠杆菌NCTC 8623倾向于从转铁蛋白中获取铁。大肠杆菌K-12菌株W3110和LG1705（如NCTC 8623）产生铁载体肠螯合素，但不产生气杆菌素，主要获得结合运铁蛋白的铁，而相关的大肠杆菌菌株LG1315和LG1628产生气杆菌素但不产生肠螯合素，表现出对源自细胞的铁的偏爱。当在59Fe标记的转铁蛋白和55Fe标记的铁蛋白存在下孵育细胞时，未观察到铁对大肠杆菌的相对利用率存在差异，这表明气杆菌素和肠螯合素从细胞内铁蛋白中去除铁的能力有所差异。不对此偏好负责。这些结果可能有助于解释为什么气杆菌素的产生，尽管它对铁的亲和力相对较低，但比肠螯合素的产生与大肠杆菌的侵袭性更紧密相关。炎症过程中细胞结合铁的可用性降低可能有助于抗菌防御。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号