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首页> 外文期刊>Infection and immunity >Association between the 65-kilodalton heat shock protein, Streptococcus sanguis, and the corresponding antibodies in Beh?et's syndrome.
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Association between the 65-kilodalton heat shock protein, Streptococcus sanguis, and the corresponding antibodies in Beh?et's syndrome.

机译：65-千洛酮热休克蛋白，血链球菌与贝氏综合征中相应的抗体之间的关联。

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The etiology of Behcet's syndrome (BS) is unknown, but a number of streptococcal species have been implicated. A hypothesis was postulated that a shared antigen, such as a stress protein, might account for some of these findings. Indeed, a rabbit antiserum against a 65-kDa heat shock protein of Mycobacterium tuberculosis revealed a corresponding 65-kDa band with all six Streptococcus sanguis strains examined and S. pyogenes but not with S. salivarius. By applying a panel of nine monoclonal antibodies to the mycobacterial 65-kDa heat shock protein, an approximately 65-kDa antigen was identified in the uncommon serotypes of S. sanguis ST3 and H.83 and one with a different Mr was identified in KTH-1 and S. pyogenes. Monoclonal antibodies Y1.2, C1.1, II H9, and ML30, which reacted with these streptococci, recognize residues 11 to 27, 88 to 123, 107 to 122, and 276 to 297 of the 65-kDa heat shock protein, respectively, suggesting that these residues are conserved among some uncommon serotypes of S. sanguis and S. pyogenes. Immunoblot analyses of sera from patients with BS for immunoglobulin A (IgA) and IgG antibodies revealed bands of 65 to 70 kDa with the mycobacterial heat shock protein, S. sanguis strains, and S. pyogenes, although these reactivities were also found to a lesser extent in controls. A 65- to 70-kDa band was found more frequently with S. sanguis KTH-2 or KTH-3 and IgA in serum from patients with BS than with serum from controls (P less than 0.02). Antibodies in serum were then studied by a radioimmunoassay, and in patients with BS this revealed significantly raised IgA antibodies to the recombinant 65-kDa mycobacterial heat shock protein and to soluble protein extracts of S. sanguis ST3, KTH-1, KTH-2, and KTH-3. Whereas significant anti-65-kDa heat shock protein and anti-S. sanguis ST3 antibodies were also found in sera from patients with rheumatoid arthritis and recurrent oral ulcers, the anti-S. sanguis KTH-1, KTH-2, and KTH-3 antibodies were confined to BS. The results are consistent with the hypothesis that some of the streptococcal antigens are associated with heat shock or stress proteins, which will need to be formally established by isolating heat shock proteins from streptococci.
机译：Behcet综合征（BS）的病因尚不明确，但涉及许多链球菌。假设一个假设是共享抗原，例如应激蛋白，可能解释了其中的一些发现。的确，针对结核分枝杆菌的65 kDa热休克蛋白的兔抗血清在所有6种链球菌血色链球菌和化脓性链球菌中均显示了一条65 kDa的条带，但唾液链球菌却没有。通过将一组九种单克隆抗体应用于分枝杆菌65 kDa热休克蛋白，可以在Sanguis ST3和H.83的罕见血清型中鉴定出大约65 kDa的抗原，而在KTH- 1和化脓性链球菌。与这些链球菌反应的单克隆抗体Y1.2，C1.1，II H9和ML30分别识别65 kDa热激蛋白的11至27、88至123、107至122和276至297残基。 ，表明这些残基在血红链霉菌和化脓链球菌的一些不常见血清型中是保守的。对BS患者血清中的免疫球蛋白A（IgA）和IgG抗体进行的免疫印迹分析显示，分枝杆菌热休克蛋白，血红链球菌菌株和化脓性链球菌具有65至70 kDa的条带，尽管这些反应性也较低。控制范围。血红假单胞菌KTH-2或KTH-3和IgA在BS患者血清中比在对照组血清中更频繁地发现65至70kDa的条带（P小于0.02）。然后，通过放射免疫分析法研究了血清中的抗体，在BS患者中，这表明针对重组65-kDa分枝杆菌热休克蛋白和桑氏葡萄球菌ST3，KTH-1，KTH-2，和KTH-3。而重要的抗65 kDa热激蛋白和抗S。在类风湿性关节炎和复发性口腔溃疡（抗S）患者的血清中也发现了sanguis ST3抗体。 sanguis KTH-1，KTH-2和KTH-3抗体仅限于BS。该结果与以下假设相符：一些链球菌抗原与热休克或应激蛋白有关，这需要通过从链球菌中分离热休克蛋白来正式建立。

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