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首页> 外文期刊>Infection and immunity >Characterization of F107 fimbriae of Escherichia coli 107/86, which causes edema disease in pigs, and nucleotide sequence of the F107 major fimbrial subunit gene, fedA.
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Characterization of F107 fimbriae of Escherichia coli 107/86, which causes edema disease in pigs, and nucleotide sequence of the F107 major fimbrial subunit gene, fedA.

机译：导致猪水肿的大肠杆菌107/86 F107菌毛的特征以及F107主要纤维亚基基因fedA的核苷酸序列。

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F107 fimbriae were isolated and purified from edema disease strain 107/86 of Escherichia coli. Plasmid pIH120 was constructed, which contains the gene cluster that codes for adhesive F107 fimbriae. The major fimbrial subunit gene, fedA, was sequenced. An open reading frame that codes for a protein with 170 amino acids, including a 21-amino-acid signal peptide, was found. The protein without the signal sequence has a calculated molecular mass of 15,099 Da. Construction of a nonsense mutation in the open reading frame of fedA abolished both fimbrial expression and the capacity to adhere to isolated porcine intestinal villi. In a screening of 28 reference edema disease strains and isolates from clinically ill piglets, fedA was detected in 24 cases (85.7%). In 20 (83.3%) of these 24 strains, fedA was found in association with Shiga-like toxin II variant genes, coding for the toxin that is characteristic for edema disease strains of E. coli. The fimbrial subunit gene was not detected in enterotoxigenic E. coli strains. Because of the capacity of E. coli HB101(pIH120) transformants to adhere to isolated porcine intestinal villi, the high prevalence of fedA in edema disease strains, and the high correlation with the Shiga-like toxin II variant toxin-encoding genes, we suggest that F107 fimbriae are an important virulence factor in edema disease strains of E. coli.
机译：从大肠杆菌的水肿病菌株107/86分离并纯化F107菌毛。构建了质粒pIH120，其包含编码粘附性F107菌毛的基因簇。对主要的纤维亚基基因fedA进行了测序。发现了一个开放阅读框，其编码具有170个氨基酸的蛋白质，包括21个氨基酸的信号肽。没有信号序列的蛋白质的计算分子量为15,099 Da。在fedA的开放阅读框中构建无意义的突变，不仅消除了纤维蛋白的表达，而且消除了其对分离的猪肠绒毛的粘附能力。从临床患病仔猪中筛选出28种参考性水肿病株和分离株，在24例中检出了fedA（85.7％）。在这24株菌株中的20株（占83.3％）中，发现fedA与志贺样毒素II变异基因相关联，该基因编码大肠杆菌水肿病株所特有的毒素。在产肠毒素的大肠杆菌菌株中未检测到纤维亚基基因。由于大肠杆菌HB101（pIH120）转化子能够粘附在分离的猪肠绒毛上，水肿病菌株中fedA的高流行性以及与志贺样毒素II变体毒素编码基因的高度相关性，我们建议F107菌毛是大肠杆菌水肿病菌株的重要毒力因子。

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