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首页> 外文期刊>Infection and immunity >Enterotoxigenicity of Vibrio parahaemolyticus with and without genes encoding thermostable direct hemolysin.
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Enterotoxigenicity of Vibrio parahaemolyticus with and without genes encoding thermostable direct hemolysin.

机译：有和没有编码热稳定直接溶血素的基因的副溶血性弧菌的肠毒力。

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Vibrio parahaemolyticus produces a thermostable direct hemolysin (TDH) that has been implicated in the pathogenesis of diarrheal disease caused by this organism. However, previous studies attempting to demonstrate the contribution of the hemolysin to virulence have been inconclusive. We investigated this putative virulence factor by using an isogenic TDH-negative (TDH-) strain constructed by specifically inactivating the two copies of the tdh gene encoding TDH. The enterotoxigenicities of the parent strain (AQ3815) and the mutant strain were tested by adding sterile culture supernatants to rabbit ileal tissue mounted in Ussing chambers. The culture filtrate of the parent strain produced a significant increase in short-circuit current (Isc), compared with the change induced by the TDH- mutant. The capacity of the culture filtrate of AQ3815 to increase the Isc was reduced by neutralization with anti-TDH serum, and the return of the cloned tdh gene to the TDH- mutant restored the ability to increase the Isc. These results were corroborated by rabbit ileal loop assays in which AQ3815 caused fluid accumulation but the TDH- mutant did not. No microscopic damage was seen in mucosal tissues exposed to the culture filtrate of either strain. These results indicate that TDH has an enterotoxigenic effect on rabbit small intestine and could be responsible for the watery diarrhea seen with V. parahaemolyticus.
机译：副溶血性弧菌产生热稳定的直接溶血素（TDH），与该生物引起的腹泻疾病的发病机理有关。但是，以前的研究试图证明溶血素对毒力的贡献尚无定论。我们通过使用通过特异性失活编码TDH的tdh基因的两个拷贝而构建的同基因TDH阴性（TDH-）菌株研究了这种推定的毒力因子。通过将无菌培养上清液添加到安装在Ussing室中的兔回肠组织中，测试了亲本菌株（AQ3815）和突变菌株的肠毒性。与由TDH-突变体引起的变化相比，亲本菌株的培养物滤液产生了短路电流（Isc）的显着增加。通过用抗TDH血清中和，降低了AQ3815的培养滤液增加Isc的能力，并且克隆的tdh基因返回TDH-突变体恢复了增加Isc的能力。这些结果通过兔回肠环测定得到了证实，其中AQ3815引起体液积聚，而TDH-突变体却没有。在暴露于任一菌株的培养物滤液的粘膜组织中均未观察到微观损伤。这些结果表明，TDH对兔小肠具有肠毒素作用，并可能导致副溶血性弧菌看到的水样腹泻。

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