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首页> 外文期刊>Infection and immunity >Homologs of Mycobacterium leprae 18-kilodalton and Mycobacterium tuberculosis 19-kilodalton antigens in other mycobacteria.
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Homologs of Mycobacterium leprae 18-kilodalton and Mycobacterium tuberculosis 19-kilodalton antigens in other mycobacteria.

机译：在其他分枝杆菌中，麻风分枝杆菌18-千达尔顿和结核分枝杆菌19-千达尔顿抗原的同系物。

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Most of the antigens of Mycobacterium leprae and M. tuberculosis that have been identified are members of stress protein families, which are highly conserved throughout many diverse species. Of the M. leprae and M. tuberculosis antigens identified by monoclonal antibodies, all except the 18-kDa M. leprae antigen and the 19-kDa M. tuberculosis antigen are strongly cross-reaction between these two species and are coded within very similar genes. Studies of T cell reactivity against mycobacterial antigens have indicated that M. tuberculosis bears epitopes that are cross-reactive with the M. leprae 18-kDa antigen, but attempts to identify an 18-kDa antigen-like protein or protein coding sequence in M. tuberculosis have been unsuccessful. We have used a combination of low-stringency DNA hybridization and polymerase chain reaction techniques to identify, isolate, and sequence genes from M. avium and M. intracellulare that are very similar to the 18-kDa antigen gene of M. leprae and others that are homologs of the 19-kDa antigen gene of M. tuberculosis. Unlike M. leprae, which contains a single 18-kDa antigen gene, M. avium and M. intracellulare each have two 18-kDa antigen coding sequences. Although the M. leprae, M. avium, and M. intracellulare 18-kDa antigen genes are all very similar to one another, as are the M. tuberculosis, M. avium, and M. intracellulare 19-kDa antigen genes, we have been unable to detect any 18-kDa antigen-like coding sequences in DNA from M. tuberculosis.
机译：已鉴定的麻风分枝杆菌和结核分枝杆菌的大多数抗原是应激蛋白家族的成员，它们在许多不同物种中高度保守。在通过单克隆抗体鉴定出的麻风分枝杆菌和结核分枝杆菌抗原中，除18 kDa麻风分枝杆菌抗原和19 kDa结核分枝杆菌抗原外，其他两个物种之间都有很强的交叉反应，并且编码在非常相似的基因中。 T细胞对分枝杆菌抗原的反应性研究表明，结核分枝杆菌具有与麻风分枝杆菌18-kDa抗原交叉反应的抗原决定簇，但试图鉴定出18.kDa抗原样蛋白或蛋白编码序列。结核病一直没有成功。我们已结合使用低严格度DNA杂交和聚合酶链反应技术来鉴定，分离和测序禽鸟支原体和胞内支原体的基因，这些基因与麻风分枝杆菌的18-kDa抗原基因非常相似，而其他是结核分枝杆菌的19-kDa抗原基因的同源物。与含有单个18-kDa抗原基因的麻风分枝杆菌不同，鸟分枝杆菌和胞内分枝杆菌分别具有两个18-kDa抗原编码序列。尽管麻风分枝杆菌，鸟分枝杆菌和胞内分枝杆菌18-kDa抗原基因彼此非常相似，结核分枝杆菌，鸟分枝杆菌和胞内分枝杆菌19-kDa抗原基因也非常相似，尚无法在结核分枝杆菌的DNA中检测到任何18-kDa抗原样编码序列。

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