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首页> 外文期刊>Infection and immunity >Pneumocystis carinii organisms obtained from rats, ferrets, and mice are antigenically different.
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Pneumocystis carinii organisms obtained from rats, ferrets, and mice are antigenically different.

机译：从大鼠，雪貂和小鼠获得的卡氏肺孢子虫微生物在抗原性上有所不同。

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Pneumocystis carinii infections were developed in animals immunosuppressed by dexamethasone treatment either from activation of latent infection (ferret) or by transtracheal inoculation with P. carinii-infected lung tissue from the homologous species (rat or mouse). Convalescent-phase antisera were obtained by stopping dexamethasone treatment after 2 to 4 weeks and allowing animals 5 to 8 weeks for recovery. P. carinii harvests from infected lungs were purified by differential filtration, solubilized in buffer containing urea, sodium dodecyl sulfate (SDS), and 2-mercaptoethanol, subjected to SDS-polyacrylamide gel electrophoresis, and blotted to polyvinylidene difluoride sheets for Western immunoblot analysis. These lung preparations are hereafter referred to as P. carinii antigens. Convalescent-phase antisera from each animal species were reacted on Western blots of P. carinii antigens prepared from organisms isolated from rats, ferrets, or mice. Each combination of P. carinii antigens and antisera from the same species of animal reacted with three or more P. carinii antigen proteins. Convalescent-phase mouse antisera reacted with P. carinii antigens from mice but not rats or ferrets. Convalescent-phase rat antisera reacted with P. carinii antigens from rats and mice but not ferrets, and convalescent-phase ferret antisera showed reactions with ferret and mouse P. carinii antigens but not rat antigens. These findings indicate antigenic differences among P. carinii strains infecting these animals.
机译：在地塞米松治疗中，由于潜伏感染的激活（雪貂）或经气管接种经卡氏假单胞菌感染的来自同种物种的肺组织（大鼠或小鼠）而受到免疫抑制的动物，发展了卡氏肺孢子虫感染。通过在2至4周后停止地塞米松治疗并让动物恢复5至8周来获得恢复期的抗血清。通过差异过滤纯化从感染肺中收获的卡氏疟原虫，将其溶于含有尿素，十二烷基硫酸钠（SDS）和2-巯基乙醇的缓冲液中，进行SDS-聚丙烯酰胺凝胶电泳，然后印迹到聚偏二氟乙烯板上进行Western免疫印迹分析。这些肺制品在下文中称为卡氏疟原虫抗原。使每种动物的恢复期抗血清在从鼠，雪貂或小鼠分离的生物体制备的卡氏疟原虫抗原的Western印迹上反应。卡氏疟原虫抗原和抗血清的每种组合与三种或更多种卡氏疟原虫抗原蛋白反应。恢复期的小鼠抗血清与小鼠的卡氏疟原虫抗原反应，而大鼠或雪貂则没有。恢复期阶段的大鼠抗血清与来自大鼠和小鼠的卡氏疟原虫抗原反应，但未与雪貂反应，而恢复期阶段的雪貂抗血清显示与雪貂和小鼠卡氏疟原虫抗原但未与大鼠抗原反应。这些发现表明感染这些动物的卡氏疟原虫菌株之间的抗原差异。

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