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首页> 外文期刊>Infection and immunity >Correlation between vacuolating cytotoxin production by Helicobacter pylori isolates in vitro and in vivo.
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Correlation between vacuolating cytotoxin production by Helicobacter pylori isolates in vitro and in vivo.

机译：幽门螺杆菌分离液在体外和体内空化细胞毒素产生之间的相关性。

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Approximately 50 to 60% of Helicobacter pylori isolates produce a vacuolating cytotoxin in vitro. To assess cytotoxin production in vivo, we sought to determine whether infection with a Tox+ H. pylori strain is associated with the presence of serum antitoxin antibodies. H. pylori isolates and serum samples were obtained from 30 patients, and serum samples were obtained from 20 uninfected patients as controls. Sera were tested by enzyme-linked immunosorbent assay for reactivity with the purified 87-kDa vacuolating cytotoxin, and the 30 H. pylori isolates were tested for vacuolating cytotoxin production. Supernatants from 14 (47%) of the 30 H. pylori isolates induced vacuolation of HeLa cells. Sera from the 30 H. pylori-infected patients reacted with the purified 87-kDa cytotoxin to a greater extent than sera from the uninfected controls for both immunoglobulin G (IgG) and IgA classes (P = 0.0004 and P < 0.0001, respectively). Serum IgG and IgA responses to the purified 87-kDa cytotoxin were higher among the 14 patients infected with Tox+ strains than among the 16 patients infected with Tox- strains (mean optical densities +/- standard errors of the means of 0.603 +/- 0.11 versus 0.234 +/- 0.07 [P = 0.005] and 0.644 +/- 0.12 versus 0.341 +/- 0.08 [P = 0.04] for IgG and IgA, respectively). Infection with a Tox+ strain compared with a Tox- strain was associated with increased antral polymorphonuclear leukocyte inflammation scores (P = 0.04). These data indicate that cytotoxin production by H. pylori isolates in vitro correlates with cytotoxin production in vivo and that infection with Tox+ H. pylori isolates may be associated with increased antral mucosal polymorphonuclear leukocyte infiltration.
机译：大约50％至60％的幽门螺杆菌分离物会在体外产生空泡细胞毒素。为了评估体内细胞毒素的产生，我们试图确定Tox +幽门螺杆菌菌株的感染是否与血清抗毒素抗体的存在有关。从30例患者中获得幽门螺杆菌分离物和血清样品，并从20例未感染患者中获得血清样品作为对照。通过酶联免疫吸附测定法检测血清与纯化的87-kDa空泡细胞毒素的反应性，并测试30株幽门螺杆菌分离物空泡细胞毒素的产生。来自30个幽门螺杆菌分离株中的14个（47％）的上清液诱导HeLa细胞空泡化。对于免疫球蛋白G（IgG）和IgA类，来自30例幽门螺杆菌感染患者的血清与纯化的87 kDa细胞毒素的反应程度大于未感染对照的血清反应（分别为P = 0.0004和P <0.0001）。在感染Tox +毒株的14例患者中，对纯化的87 kDa细胞毒素的血清IgG和IgA的反应要高于感染Tox-毒株的16例患者（平均光密度+/-标准误差的平均值为0.603 +/- 0.11 IgG和IgA分别为0.234 +/- 0.07 [P = 0.005]和0.644 +/- 0.12与0.341 +/- 0.08 [P = 0.04]。与Tox-毒株相比，Tox +毒株的感染与窦性多形核白细胞炎症评分增加有关（P = 0.04）。这些数据表明，幽门螺杆菌分离株在体外的细胞毒素产生与体内细胞毒素的产生相关，而Tox +幽门螺杆菌分离株的感染可能与肛门黏膜多形核白细胞浸润的增加有关。

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