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首页> 外文期刊>Infection and immunity >Cryptosporidium muris in adult mice: adoptive transfer of immunity and protective roles of CD4 versus CD8 cells.
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Cryptosporidium muris in adult mice: adoptive transfer of immunity and protective roles of CD4 versus CD8 cells.

机译：成年小鼠中的隐孢子虫：CD4与CD8细胞的免疫力过继转移和保护作用。

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The aim of this study was to investigate the role of the CD4 and CD8 T cells in immunity to cryptosporidia by using Cryptosporidium muris and a mouse model of infection. Two approaches were used, each involving the use of rat anti-T-cell surface marker monoclonal antibodies (MAbs). In the first, the adoptive transfer of immunity was studied by using the CB.17 SCID mouse (which lacks T and B cells) as the host; in the second, the effect on susceptibility of BALB/c mice to infection was examined following depletion of T cells or subsets of T cells. In adoptive immunity experiments, the conditions which differentiated between resistance associated with reconstitution of SCID mice with naive BALB/c lymphocytes and the transfer of immunity with primed lymphocytes from infected animals were determined. Primed spleen or mesenteric lymph node cells conferred better protection to recipients than naive cells when obtained from donors which had developed resistance to infection. Adoptive immunity was abrogated when Thy.1 cells or CD4 cells were depleted from primed cells, while depletion of CD8 cells could reduce the level of protection. In the study of C. muris in BALB/c mice, treatment with either anti-Thy.1 plus anti-Lyt.1 or anti-CD4 MAbs increased susceptibility to a primary infection as determined by the size and duration of oocyst production, but an anti-CD8 MAb produced an increase only in oocyst shedding. Thus, both CD4 and, to a lesser extent, CD8 cells appeared to be involved in resistance to primary and secondary C. muris infection.
机译：这项研究的目的是通过使用鼠隐孢子虫和小鼠感染模型研究CD4和CD8 T细胞在对隐孢子虫免疫中的作用。使用了两种方法，每种方法都涉及使用大鼠抗T细胞表面标志物单克隆抗体（MAb）。首先，以CB.17 SCID小鼠（缺乏T细胞和B细胞）为宿主，研究了免疫的过继转移。第二，在耗尽T细胞或T细胞亚群后，检查了对BALB / c小鼠感染的敏感性。在过继免疫实验中，确定了区分与用原始BALB / c淋巴细胞重建SCID小鼠的抵抗力和来自感染动物的初免淋巴细胞转移免疫力的条件。从脾脏或肠系膜淋巴结初免细胞中获得的保护性要比从已对感染产生抵抗力的供体中获得的幼稚细胞更好。当Thy.1细胞或CD4细胞从初免细胞中耗竭时，过继免疫被取消，而CD8细胞的耗竭会降低保护水平。在BALB / c小鼠中的鼠毛衣原体研究中，用抗Thy.1加上抗Lyt.1或抗CD4 MAb的治疗增加了对原发感染的敏感性，这取决于卵囊产生的大小和持续时间，但是抗CD8 MAb仅增加卵囊脱落。因此，CD4细胞和CD8细胞在较小程度上似乎都参与了对原发和继发梭状芽孢杆菌感染的抗性。

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