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首页> 外文期刊>Infection and immunity >Relationship of Impairment of Schistosome 28-Kilodalton Glutathione S-Transferase (GST) Activity to Expression of Immunity to Schistosoma mattheei in Calves Vaccinated with Recombinant Schistosoma bovis28-Kilodalton GST
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Relationship of Impairment of Schistosome 28-Kilodalton Glutathione S-Transferase (GST) Activity to Expression of Immunity to Schistosoma mattheei in Calves Vaccinated with Recombinant Schistosoma bovis28-Kilodalton GST

机译：牛重组牛血吸虫28-Kilodalton GST疫苗接种后犊牛血吸虫28-Kilodalton谷胱甘肽S-转移酶（GST）活性受损与免疫力表达的关系

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Sera from calves vaccinated with the recombinant Schistosoma bovis-derived 28-kDa glutathione S-transferase (28GST) and subsequently naturally or experimentally exposed toSchistosoma mattheei were studied for their content of specific immunoglobulin G (IgG) and IgA antibodies to recombinantS. bovis 28GST as well as for their capacity to inhibit the enzymatic activity of the antigen. The results were analyzed in regard to the presence (natural infection) or absence (experimental infection) of a protective effect(s) (reductions in worm burden, egg load, fecal egg counts, and excretion of viable eggs) toward S. mattheei challenge. Under such conditions, no differences in the IgG- and IgA-specific antibodies to recombinant S. bovis28GST or in the ability to block the catalytic function of the antigen between the two groups were recorded. Nevertheless, correlation analysis between the specific antibody responses to recombinantS. bovis 28GST and the inhibition of GST activity suggested an association with IgG in experimentally infected vaccinated animals, while in naturally infected vaccinated calves, the inhibitory activity appeared to be linked to a greater degree with IgA. These results suggest that in contrast to schistosomiasis in humans, IgG antibodies in calves with schistosomiasis may exhibit inhibitory functions toward GST enzymatic activity or have a modulatory effect on IgA antibody properties. Furthermore, sera from animals immunized with recombinantS. bovis 28GST recognized the native S. mattheei 28GST and achieved comparable levels of inhibition of activity of recombinant S. bovis 28GST and S. matthei 28GST, indicating the presence of cross-reactive epitopes on these two molecules.
机译：用重组牛源血吸虫 S -转移酶（28GST）接种了牛重组血吸虫的小牛血清，随后自然或实验暴露于马氏血吸虫研究了它们对重组em S的特异性免疫球蛋白G（IgG）和IgA抗体的含量。 bovis 28GST及其抑制抗原酶活性的能力。分析结果是否存在（自然感染）或不存在（实验性感染）（对 S）具有保护作用（减少蠕虫负担，减少卵负荷，粪便卵数和排出活卵） 。 mattheei 挑战。在这种条件下，针对重组 S的IgG和IgA特异性抗体没有差异。记录了Bovis 28GST或在两组之间阻断抗原催化功能的能力。然而，对重组em S的特异性抗体反应之间的相关性分析。牛28 GST和对GST活性的抑制表明在实验感染的接种疫苗的动物中IgG与IgG有关，而在自然感染的接种小牛中，抑制活性似乎与IgA的关联更大。这些结果表明，与人类的血吸虫病相反，患有血吸虫病的牛犊中的IgG抗体可能对GST酶活性具有抑制作用，或者对IgA抗体的性质具有调节作用。此外，用重组em S免疫的动物血清。 bovis 28GST识别了本地的 S。 mattheei 28GST并达到了可比的重组 S活性抑制水平。 bovis 28GST和 S。 matthei 28GST，表明这两个分子上存在交叉反应性表位。

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