• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Age-Dependent Neisseria meningitidis Serogroup C Class-Specific Antibody Concentrations and Bactericidal Titers in Sera from Young Children from Montana Immunized with a Licensed Polysaccharide Vaccine
【24h】

Age-Dependent Neisseria meningitidis Serogroup C Class-Specific Antibody Concentrations and Bactericidal Titers in Sera from Young Children from Montana Immunized with a Licensed Polysaccharide Vaccine

机译：蒙大拿州幼童血清中年龄依赖性脑膜炎奈瑟氏球菌血清C类特定抗体浓度和杀菌滴度的免疫，接种了许可的多糖疫苗

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Neisseria meningitidis serogroup C bactericidal titers and class-specific enzyme-linked immunosorbent assay (ELISA) antibody concentrations were measured in sera from 173 children (1 to 5 years old) before and 6 weeks and 7 months following vaccination with a quadrivalent (A/C/Y/W-135) polysaccharide vaccine. The immune responses of the children were compared with those of 40 adults 6 weeks postvaccination. Both bactericidal titers and ELISA antibody concentrations were significantly higher in the adults than in the children (P < 0.05). In addition, the ratio of immunoglobulin G (IgG) to IgM was higher in the children than in the adults. With an ELISA total antibody concentration of ≥2 μg/ml used as a measure of seroconversion, ≥84% of the individuals from each age group responded to the serogroup C polysaccharide. However, with a ≥4-fold-increase in bactericidal titer used, only 18% of 1-year-olds, 32% of 2-year-olds, and 50 to 60% of 3-, 4-, and 5-year-olds seroconverted. The ELISA results suggest that >50% of all children retained ≥2 μg of total antibody per ml at 7 months postimmunization. However, the bactericidal titers suggest that <10% of children <4 years old retained a ≥4-fold increase at 7 months following vaccination. Of particular note, 59 of 79 sera (75%) from the 1- and 2-year-olds had high ELISA antibody concentrations (2 to 20 μg/ml) with no associated bactericidal titer (<1:8). Discordant results between bactericidal titers and ELISA antibody concentrations were not explained by the presence of IgA blocking antibody or relative levels of IgG and IgM. The bactericidal results show age-dependent differences in the production and retention of antibody in young children immunized with serogroup C polysaccharide; these differences are not evident with the ELISA data.
机译：在接种疫苗之前，接种后6周和7个月的173名儿童（1至5岁）的血清中测定了脑膜炎奈瑟菌（Neemeria meningitidis）血清群C的杀菌效价和类特异性酶联免疫吸附测定（ELISA）抗体浓度用四价（A / C / Y / W-135）多糖疫苗接种。将儿童的免疫反应与接种疫苗后6周的40位成人的免疫反应进行了比较。成人的杀菌滴度和ELISA抗体浓度均显着高于儿童（ P <0.05）。此外，儿童的免疫球蛋白G（IgG）与IgM的比例高于成人。使用ELISA总抗体浓度≥2μg/ ml作为血清转换的量度，每个年龄组的≥84％的个体对血清C多糖有反应。但是，如果使用的杀菌滴度提高了4倍以上，则1岁儿童中只有18％，2岁儿童中只有32％，3、4和5岁儿童中有50至60％ -seros转换了。 ELISA结果表明，免疫后7个月，> 50％的儿童每毫升保留总抗体≥2μg。但是，杀菌效价表明，<4％的儿童中，<10％的儿童在接种疫苗后7个月保持了≥4倍的增长。特别值得注意的是，来自1岁和2岁儿童的79个血清中有59个（75％）具有较高的ELISA抗体浓度（2至20μg/ ml），没有相关的杀菌效价（<1：8）。杀菌效价和ELISA抗体浓度之间的不一致结果无法通过IgA封闭抗体的存在或IgG和IgM的相对水平来解释。杀菌结果显示，用血清群C多糖免疫的幼儿的抗体产生和保留的年龄依赖性差异;这些差异在ELISA数据中并不明显。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号