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首页> 外文期刊>Infection and immunity >Characterization of monoclonal antibodies to heat-labile enterotoxin encoded by a plasmid from a clinical isolate of Escherichia coli.
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Characterization of monoclonal antibodies to heat-labile enterotoxin encoded by a plasmid from a clinical isolate of Escherichia coli.

机译：表征抗热不稳定肠毒素的单克隆抗体的抗体，该毒素由大肠杆菌临床分离株中的质粒编码。

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Eight selected hybridoma cell lines that produced monoclonal antibodies against heat-labile enterotoxin from an Escherichia coli strain of human origin (LTh) were characterized. Antibodies produced by these cell lines were tested for binding specificity in a series of solid-phase radioimmunoassays and Western blots by using as test antigens LTh, the A, A1, A2, and B polypeptides of LTh, the heat-labile enterotoxin from an E. coli strain of porcine origin, and cholera toxin. The monoclonal antibodies were also tested for isotype and ability to neutralize LTh. Two of the anti-LTh monoclonal antibodies cross-reacted with cholera toxin, and six were specific for determinants of LTh that were not present on cholera toxin. One was specific for a unique epitope of LTh that was not shared by the heat-labile enterotoxin from an E. coli strain of porcine origin or cholera toxin. Four antibodies specific for epitopes on the B subunit of LTh (LTh-B) reacted with pentameric LTh-B but did not react in Western blots with monomeric LTh-B. The remaining four antibodies were specific for epitopes on LTh-A; two of these antibodies bound to A1, one reacted with A2, and one recognized only intact LTh-A. Only one monoclonal antibody had detectable neutralizing activity, and it was specific for LTh-A.
机译：对八种选择的杂交瘤细胞系进行了表征，这些细胞系从人源大肠杆菌菌株（LTh）产生了抗热不稳定肠毒素的单克隆抗体。在一系列固相放射免疫分析和蛋白质印迹中，通过使用LTh，LTh的热不稳定肠毒素Lth，A，A1，A2和B多肽作为测试抗原，对这些细胞系产生的抗体的结合特异性进行了测试。猪源性大肠杆菌菌株和霍乱毒素。还测试了单克隆抗体的同种型和中和LTh的能力。抗LTh单克隆抗体中的两种与霍乱毒素交叉反应，六种对霍乱毒素中不存在的LTh决定簇具有特异性。一种是特异于LTh的独特表位，而猪源性或霍乱毒素的大肠杆菌菌株的不耐热肠毒素却不共享这种独特的LTh表位。特异于LTh B亚基（LTh-B）上的表位的四种抗体与五聚体LTh-B反应，但在Western blot中未与单体LTh-B反应。其余四种抗体对LTh-A上的表位具有特异性。其中两种抗体与A1结合，一种与A2反应，另一种仅识别完整的LTh-A。只有一种单克隆抗体具有可检测的中和活性，并且对LTh-A具有特异性。

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