• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Phorbol myristate acetate-induced modulation of antibody-dependent cellular cytotoxicity by human polymorphonuclear leukocytes.
【24h】

Phorbol myristate acetate-induced modulation of antibody-dependent cellular cytotoxicity by human polymorphonuclear leukocytes.

机译：佛波肉豆蔻酸酯乙酸盐诱导的人多形核白细胞对抗体依赖性细胞毒性的调节。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The effect of phorbol myristate acetate (PMA) on the antibody-dependent cellular cytotoxicity (ADCC) of human polymorphonuclear leukocytes (PMNs) against human erythroleukemic K562 cells was studied by the use of a 3-h 51Cr-release assay. Pretreatment of PMNs with PMA (10 ng/ml) for 60 min resulted in inhibition of subsequent ADCC. This inhibition was dependent on doses of PMA. The effect of pretreatment of PMNs with PMA on O2- generation of the cells was also studied. The ability of the cells to generate O2- was not suppressed, and the expression of Fc receptors on the cell membrane was well preserved. In contrast, the addition of PMA to the ongoing ADCC (5 to 30 min after the start of the ADCC assay) enhanced the activity of the cells for ADCC. This augmentation was abolished by catalase, whereas ADCC itself was not affected by the agent. These results imply divalent effects of PMA on the ADCC of PMNs. The suppression of ADCC activity of PMNs by pretreatment with PMA is not due to inhibition of the reactive oxygen burst of the cells. The augmentation of ongoing ADCC by the addition of PMA is due to secretion of hydrogen peroxide from the cells induced by PMA, and this augmentation occurs only when the interaction between effector and target cells exists through Fc receptor.
机译：通过使用3小时51Cr释放试验研究了佛波肉豆蔻酸酯乙酸酯（PMA）对人多形核白细胞（PMN）对人红白血病K562细胞的抗体依赖性细胞毒性（ADCC）的影响。用PMA（10 ng / ml）预处理PMN 60分钟导致抑制后续ADCC。这种抑制取决于PMA的剂量。还研究了用PMA预处理PMN对细胞O2生成的影响。细胞产生O 2的能力没有被抑制，并且Fc受体在细胞膜上的表达得到了很好的保留。相反，向进行中的ADCC中添加PMA（ADCC分析开始后5至30分钟）可增强细胞对ADCC的活性。过氧化氢酶消除了这种增加，而ADCC本身不受该试剂的影响。这些结果暗示PMA对PMN的ADCC具有二价作用。用PMA预处理抑制PMN的ADCC活性不是由于抑制了细胞的活性氧爆发。通过添加PMA来进行中的ADCC的增加是由于PMA诱导的细胞中过氧化氢的分泌，并且这种增加仅在效应子和靶细胞之间通过Fc受体存在相互作用时才发生。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号