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首页> 外文期刊>Infection and immunity >Characterization of antibody to the ferripyochelin-binding protein of Pseudomonas aeruginosa.
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Characterization of antibody to the ferripyochelin-binding protein of Pseudomonas aeruginosa.

机译：铜绿假单胞菌的ferripyochelin结合蛋白抗体的表征。

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An outer membrane protein of Pseudomonas aeruginosa was previously shown to bind 59Fe-labeled pyochelin. Antibodies to the purified ferripyochelin-binding protein (FBP) were characterized by using a variety of assays. Anti-FBP cross-reacted with several P. aeruginosa isolates in an enzyme-linked immunosorbent assay. Anti-FBP significantly enhanced phagocytosis of P. aeruginosa by human polymorphonuclear leukocytes. In a serum bactericidal assay we observed no difference in viability between cells incubated with antiserum to FBP and cells incubated with preimmune serum. Anti-FBP immunoglobulin G inhibited both binding and uptake of 59Fe-labeled pyochelin by whole cells. Passive protection by anti-FBP was examined in experimental P. aeruginosa burn infections in mice. The protection provided by this antibody was strain dependent but lipopolysaccharide serotype independent.
机译：先前显示铜绿假单胞菌的外膜蛋白可结合59Fe标记的pyochelin。通过使用多种测定来表征纯化的铁结合蛋白的结合蛋白（FBP）的抗体。在酶联免疫吸附试验中，抗FBP与几种铜绿假单胞菌分离物发生交叉反应。抗FBP显着增强人多形核白细胞对铜绿假单胞菌的吞噬作用。在血清杀菌试验中，我们观察到与FBP抗血清孵育的细胞与免疫前血清孵育的细胞之间的生存力没有差异。抗FBP免疫球蛋白G抑制全细胞对59Fe标记的秋水仙素的结合和摄取。在小鼠的实验性铜绿假单胞菌烧伤感染中检查了抗FBP的被动保护作用。该抗体提供的保护是菌株依赖性的，但脂多糖血清型无关。

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