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首页> 外文期刊>Infection and immunity >Correlation of natural killer cell activity and clearance of Cryptococcus neoformans from mice after adoptive transfer of splenic nylon wool-nonadherent cells.
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Correlation of natural killer cell activity and clearance of Cryptococcus neoformans from mice after adoptive transfer of splenic nylon wool-nonadherent cells.

机译：过继转移脾脏尼龙毛-非粘附细胞后，小鼠自然杀伤细胞活性与新隐球菌清除率的相关性。

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Previous reports demonstrate that natural killer (NK) cells inhibit the growth of Cryptococcus neoformans in vitro, but conclusive evidence supporting the effectiveness of NK cells in host resistance to cryptococci is not available. The objective of these studies was to assess the ability of NK cells to clear C. neoformans from the lungs, livers, and spleens of infected mice. CBA/J mice were depleted of NK cells, as well as other natural effector cells, by an intraperitoneal injection of cyclophosphamide (Cy), 240 mg/kg of body weight. One day later, 7.5 X 10(7) nylon wool-nonadherent (NWN) spleen cells, either untreated or treated with anti-asialo GM1 and complement to remove NK cells, were adoptively transferred to Cy-pretreated mice. On day 2 after Cy treatment, the mice were injected intravenously with 2 X 10(4) cryptococci. At 4 and 6 days after Cy treatment, tissues were assayed for NK reactivity, using a 4-h 51Cr-release assay, and for in vivo clearance of cryptococci as reflected by mean log10 CFU per organ. We observed that Cy treatment depleted NK activity against YAC-1 targets and reduced in vivo clearance of C. neoformans from the tissues of infected mice. Additionally, Cy treatment depleted the total lung and spleen cellularity and the total number of peripheral blood lymphocytes when compared with those in normal untreated control mice. Also, spleen weights were significantly decreased in comparison with those of untreated animals 4 days after Cy treatment. Adoptive transfer of untreated NWN spleen cells into Cy-depressed mice restored the NK cell activity which correlated with enhanced clearance of cryptococci from lungs, livers, and spleens. In contrast, treatment of NWN spleen cells with anti-asialo GM1 and complement before adoptive transfer abrogated the ability of these cells to restore NK activity or reduce the numbers of cryptococci present in tissues of infected mice. Taken together, these data indicate that NK cells are the cells effective in diminishing the numbers of cryptococci in tissues of infected mice. Consequently, NK cells may play a role in first-line host resistance against C. neoformans.
机译：先前的报道表明，自然杀伤（NK）细胞在体外可抑制新型隐球菌的生长，但尚无确凿的证据支持NK细胞在宿主对隐球菌的抗性中的有效性。这些研究的目的是评估NK细胞清除被感染小鼠的肺，肝和脾脏中新形成梭状芽胞杆菌的能力。通过腹膜内注射体重240 mg / kg的环磷酰胺（Cy），使CBA / J小鼠的NK细胞以及其他自然效应细胞耗尽。一天后，将未经处理或已用抗亚细亚蓝GM1处理并用补体去除NK细胞的7.5 X 10（7）尼龙羊毛非粘附（NWN）脾细胞过继转移至Cy预处理的小鼠中。 Cy处理后第2天，给小鼠静脉注射2 X 10（4）隐球菌。 Cy处理后第4天和第6天，使用4-h 51Cr释放测定法测定组织的NK反应性，并通过每个器官的平均log10 CFU反映隐球菌的体内清除率。我们观察到Cy处理可减少针对YAC-1靶的NK活性，并减少新感染梭状芽胞杆菌从感染小鼠组织中的体内清除率。另外，与正常未治疗的对照小鼠相比，Cy治疗消耗了总的肺和脾细胞的细胞性以及外周血淋巴细胞的总数。此外，与Cy处理后4天未治疗的动物相比，脾脏的重量显着降低。未处理的NWN脾细胞过继转移到Cy抑制的小鼠中恢复了NK细胞活性，这与隐球菌从肺，肝和脾中清除的增强有关。相比之下，在过继转移之前用抗亚洲人的GM1和补体处理NWN脾细胞，则废除了这些细胞恢复NK活性或减少感染小鼠组织中存在的隐球菌数量的能力。综上所述，这些数据表明NK细胞是有效减少感染小鼠组织中隐球菌数量的细胞。因此，NK细胞可能在一线宿主对新孢梭菌的抗性中发挥作用。

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