Incorporation of the major outer membrane protein of Neisseria gonorrhoeae in saponin-lipid complexes (iscoms): chemical analysis, some structural features, and comparison of their immunogenicity with three other antigen delivery systems.

G F Kersten; T Teerlink; H J Derks; A J Verkleij; T L van Wezel; D J Crommelin; E C Beuvery

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Incorporation of the major outer membrane protein of Neisseria gonorrhoeae in saponin-lipid complexes (iscoms): chemical analysis, some structural features, and comparison of their immunogenicity with three other antigen delivery systems.

机译：将淋病奈瑟氏球菌的主要外膜蛋白掺入皂素-脂质复合物（iscoms）中：化学分析，一些结构特征，并将其免疫原性与其他三种抗原递送系统进行比较。

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We incorporated the major outer membrane protein (PI) of Neisseria gonorrhoeae into immunostimulating complexes (iscoms) and examined some analytical, physicochemical, and immunological properties of these structures. The immunogenicity was compared with that of three other PI-containing structures, i.e., liposomes, outer membrane complexes produced by the bacterium, and protein-detergent-adjuvant complexes. AIPO4 and dioctadecyldimethylammonium bromide were used as adjuvants. Our results show that iscoms are much more immunogenic than liposomes and protein-detergent complexes but are also much more toxic. The localization of PI in iscoms was investigated. Therefore, the chymotrypsin susceptibility of PI in iscoms was tested, and the incorporation of fragments of PI was determined. Amphiphilic fragments of PI were incorporated in iscoms, but hydrophilic and hydrophobic fragments were not. Chymotrypsin degradation of PI in iscoms indicated that the protein is exposed to the environment in a similar manner as PI in outer membrane complexes, i.e., with both termini anchored in the iscom.

机译：我们将淋病奈瑟氏球菌的主要外膜蛋白（PI）纳入了免疫刺激复合物（iscoms），并检查了这些结构的一些分析，理化和免疫学性质。将该免疫原性与其他三种含PI的结构，即脂质体，细菌产生的外膜复合物和蛋白质-洗涤剂-佐剂复合物的免疫原性进行了比较。将AIPO4和二十八烷基二甲基溴化铵用作佐剂。我们的结果表明，iscoms比脂质体和蛋白质洗涤剂复合物具有更高的免疫原性，但毒性也更高。研究了PI在iscoms中的定位。因此，测试了胰蛋白酶在胰岛胰蛋白酶中对胰凝乳蛋白酶的敏感性，并确定了PI片段的掺入。 PI的两亲性片段被并入iscoms中，而亲水性和疏水性片段则没有。胰凝乳蛋白酶对iscoms中PI的降解表明该蛋白以与外膜复合物中的PI相似的方式暴露于环境，即两个末端均锚定在iscom中。

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《Infection and immunity》 |1988年第2期|共7页
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G F Kersten; T Teerlink; H J Derks; A J Verkleij; T L van Wezel; D J Crommelin; E C Beuvery;
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中图分类医学免疫学;
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入库时间 2022-08-18 18:24:24

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1. Immunogenicity of liposomes and iscoms containing the major outer membrane protein of Neisseria gonorrhoeae: influence of protein content and liposomal bilayer composition. [J] . G F Kersten, A M van de Put, T Teerlink, Infection and immunity . 1988,第6期

机译：淋病奈瑟氏球菌主要外膜蛋白的脂质体和iscoms的免疫原性：蛋白含量和脂质体双层组成的影响。
2. Structural comparison of Neisseria gonorrhoeae outer membrane proteins. [J] . J E Heckels Journal of bacteriology . 1981,第2期

机译：淋病奈瑟氏球菌外膜蛋白的结构比较。
3. The major anaerobically induced outer membrane protein of Neisseria gonorrhoeae, Pan 1, is a lipoprotein. [J] . G T Hoehn, V L Clark Infection and immunity . 1992,第11期

机译：淋病奈瑟氏球菌的主要厌氧诱导的外膜蛋白Pan 1是一种脂蛋白。
4. Structural analysis of protein complexes by chemical cross-linking and mass spectrometry [C] . Alexander Leitner, Florian Stengel, Thomas Walzthoeni, International Mass Spectrometry Conference . 2014

机译：化学交联和质谱法的蛋白质复合物的结构分析
5. Assessing the immunogenicity of the major outer membrane protein Porin B of Neisseria gonorrhoeae as a vaccine candidate. [D] . Le, TuQuynh. 2014

机译：评估淋病奈瑟氏球菌主要外膜蛋白Porin B作为候选疫苗的免疫原性。
6. Incorporation of the major outer membrane protein of Neisseria gonorrhoeae in saponin-lipid complexes (iscoms): chemical analysis some structural features and comparison of their immunogenicity with three other antigen delivery systems. [O] . G F Kersten, T Teerlink, H J Derks, 1988

机译：将淋病奈瑟氏球菌的主要外膜蛋白掺入皂素-脂质复合物（iscoms）中：化学分析一些结构特征以及与其他三种抗原递送系统的免疫原性比较。
7. Incorporation of the major outer membrane protein of Neisseria gonorrhoeae in saponin-lipid complexes (iscoms): chemical analysis, some structural features, and comparison of their immunogenicity with three other antigen delivery systems [O] . G F Kersten, T Teerlink, H J Derks, 1988

机译：在皂苷 - 脂质复合物（ISCOMS）中掺入Neisseria淋病术的主要外膜蛋白：化学分析，一些结构特征，以及与三种其他抗原输送系统的免疫原性的比较
8. Type-Specific Antigens of Group A Neisseria meningitidis: Lipopolysaccharide and Heat-Modifiable Outer Membrane Proteins [R] . Zollinger, W. D., Mandrell, R. E. 1980

机译：a群脑膜炎奈瑟菌的类型特异性抗原：脂多糖和可热改性的外膜蛋白

Incorporation of the major outer membrane protein of Neisseria gonorrhoeae in saponin-lipid complexes (iscoms): chemical analysis, some structural features, and comparison of their immunogenicity with three other antigen delivery systems.

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