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首页> 外文期刊>Infection and immunity >Immunobiological activities of a porin fraction isolated from Fusobacterium nucleatum ATCC 10953.
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Immunobiological activities of a porin fraction isolated from Fusobacterium nucleatum ATCC 10953.

机译：从核梭状芽胞杆菌ATCC 10953中分离出的孔蛋白级分的免疫生物学活性。

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摘要

From Fusobacterium nucleatum ATCC 10953 cell envelope fraction whose inner membranes had been removed by treatment with sodium N-lauroyl sarcosinate, an outer membrane protein (37,000 Mr in a native state) was prepared by extraction with lithium dodecyl sulfate. The protein thus obtained showed distinct porin activity, namely, the ability to form hydrophilic diffusion pores by incorporation into the artificial liposome membrane. The porin fraction exhibited strong immunobiological activities in the in vitro assays: B-cell mitogenicity and polyclonal B-cell activation on murine splenocytes, stimulatory effects on guinea pig peritoneal macrophages, and enhancement of the migration of human blood monocytes. The porin fraction also exhibited immunoadjuvant activity to increase the antibody production against sheep erythrocytes in the spleen of mice that were immunized by sheep erythrocytes with porin. Although chemical analyses revealed that the test porin fraction contained a considerable amount of lipopolysaccharide (LPS) (around 12% of the fraction), the studies with LPS-nonresponding C3H/HeJ mice and on the inhibitory effects of polymyxin B strongly suggest that most of the above bioactivities are due to porin protein itself, not to coexistent LPS in the porin fraction.
机译：通过用N-月桂酰肌氨酸钠处理除去内膜后的核梭状芽胞杆菌ATCC 10953细胞被膜部分，用十二烷基硫酸锂萃取制备外膜蛋白（37,000Mr，天然状态）。如此获得的蛋白质显示出明显的孔蛋白活性，即通过掺入人工脂质体膜而形成亲水性扩散孔的能力。孔蛋白级分在体外试验中表现出强大的免疫生物学活性：对小鼠脾细胞的B细胞有丝分裂性和多克隆B细胞活化，对豚鼠腹膜巨噬细胞的刺激作用以及人类血单核细胞迁移的增强。孔蛋白级分还表现出免疫佐剂活性，以增加用孔蛋白对绵羊红细胞免疫的小鼠脾脏中针对绵羊红细胞的抗体产生。尽管化学分析显示受试孔蛋白级分含有大量脂多糖（LPS）（约占该级分的12％），但对LPS无反应的C3H / HeJ小鼠以及多粘菌素B的抑制作用的研究强烈表明，大多数上述生物活性是由于孔蛋白本身，而不是孔蛋白级分中的LPS共存所致。

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