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首页> 外文期刊>Infection and immunity >Immunoserological comparison of 104-kilodalton proteins associated with hemolysis and cytolysis in Actinobacillus pleuropneumoniae, Actinobacillus suis, Pasteurella haemolytica, and Escherichia coli.
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Immunoserological comparison of 104-kilodalton proteins associated with hemolysis and cytolysis in Actinobacillus pleuropneumoniae, Actinobacillus suis, Pasteurella haemolytica, and Escherichia coli.

机译：与胸膜肺炎放线杆菌，猪胃放线杆菌，溶血巴斯德氏菌和大肠杆菌中的溶血和细胞溶解有关的104千道尔顿蛋白的免疫血清学比较。

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A homologous polyclonal antibody was produced in a rabbit to the 104-kilodalton (kDa) protein hemolysin of Actinobacillus pleuropneumoniae serotype 1 strain CM-5. In immunoblots, this antibody recognized a similar 104-kDa protein produced in culture supernatants by A. pleuropneumoniae serotypes 1 to 12 and taxon "Minor group" in addition to Pasteurella haemolytica, Actinobacillus suis, and alpha-hemolysin-producing Escherichia coli (but only weakly in the latter two organisms). These results were reproduced by using a mouse monoclonal antibody to the CM-5 104-kDa protein hemolysin, except that the monoclonal antibody bound more strongly to the alpha-hemolysin produced by E. coli, only weakly to the 104-kDa protein produced by "Minor group," and not at all to any extracellular antigens produced by A. suis. Pigs experimentally infected with A. pleuropneumoniae serotypes 1 to 10 and A. suis produced an antibody that recognized the 104-kDa hemolysin produced by CM-5. A pig challenged with a "Minor group" strain did not have such antibodies. Rabbit antiserum produced against the leukotoxin of P. haemolytica and alpha-hemolysin-producing E. coli also recognized the CM-5 hemolysin, but the latter only weakly. The hemolytic activity produced by CM-5 in culture supernatant was neutralized strongly by the pig serum to serotypes 1, 2, 5, 6, 9, and 10 and A. suis, only partially by serotype 8 antiserum and the rabbit antiserum to P. haemolytica leukotoxin, and not all by the antiserum to serotypes 3, 4, and 7 and "Minor group" and the E. coli alpha-hemolysin. These results indicate that a similar but not identical 104-kDa protein is produced in vitro and in vivo by all serotypes of A. pleuropneumoniae and may be related to cytolysins produced by other gram-negative bacteria.
机译：在兔中产生了针对胸膜肺炎放线杆菌血清型1菌株CM-5的104千达尔顿（kDa）蛋白溶血素的同源多克隆抗体。在免疫印迹中，此抗体可识别胸膜肺炎链球菌血清型1至12和分类群“次要组”在培养上清液中产生的类似的104 kDa蛋白，除了溶血巴斯德氏菌，猪放线杆菌和产α-溶血素的大肠杆菌（但仅在后两种生物中微弱）。这些结果是通过使用针对CM-5 104-kDa蛋白溶血素的小鼠单克隆抗体重现的，除了该单克隆抗体与大肠杆菌产生的α-溶血素的结合更强，而与由大肠杆菌产生的104-kDa蛋白的结合较弱。 “次要基团”，而不是猪链球菌产生的任何细胞外抗原。实验上感染了胸膜肺炎链球菌血清型1至10和猪链球菌的猪产生了识别CM-5产生的104 kDa溶血素的抗体。用“次要组”菌株攻击的猪不具有此类抗体。针对溶血性疟原虫的白细胞毒素和产生α-溶血素的大肠杆菌产生的兔抗血清也识别出CM-5溶血素，但后者仅是弱的。 CM-5在培养上清液中产生的溶血活性被猪血清强烈中和至血清型1,2,5,6,9,10和猪链球菌，仅部分被血清型8抗血清和兔抗P.溶血性白细胞毒素，并且并非全部通过针对血清型3、4和7的抗血清以及“次要组”和大肠杆菌α-溶血素来实现。这些结果表明，胸膜肺炎链球菌的所有血清型在体外和体内均产生相似但不相同的104 kDa蛋白，并且可能与其他革兰氏阴性细菌产生的溶细胞素有关。

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