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首页> 外文期刊>Infection and immunity >Cloning and characterization of Treponema hyodysenteriae antigens and protection in a CF-1 mouse model by immunization with a cloned endoflagellar antigen.
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Cloning and characterization of Treponema hyodysenteriae antigens and protection in a CF-1 mouse model by immunization with a cloned endoflagellar antigen.

机译：猪痢疾密螺旋体抗原的克隆，鉴定和在CF-1小鼠模型中的保护作用是通过克隆的鞭毛内抗原进行免疫。

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We cloned genes that code for Treponema hyodysenteriae antigens into Escherichia coli with the purpose of identifying protective antigens for vaccine development. Three different genomic libraries were screened with various antisera reactive with T. hyodysenteriae antigens. The cloned antigens and corresponding native T. hyodysenteriae antigens were analyzed for molecular size, serum reactivity, solubility in sarcosine, and segregation during phase partitioning with the nonionic detergent Triton X-114. The results from these analyses suggested that the gene products were components of either the cytoplasmic membrane, periplasm, or endoflagella of T. hyodysenteriae. The cloned antigens were tested as vaccine candidates in a CF-1 mouse model of T. hyodysenteriae infection and immunity. Intraperitoneal injection of crude E. coli extracts containing cloned antigens did not protect mice from challenge. However, serum from mice injected with a crude extract of an E. coli clone which expressed an endoflagellar antigen killed T. hyodysenteriae in vitro. Partially purified preparations of this cloned endoflagellar antigen protected mice against oral challenge with both the homologous serotype (B204) and a heterologous serotype (B234) of T. hyodysenteriae. These results suggest that the endoflagellar proteins could be used as an effective subunit vaccine against T. hyodysenteriae.
机译：我们将编码猪痢疾密螺旋体抗原的基因克隆到大肠杆菌中，以鉴定用于疫苗开发的保护性抗原。用与猪痢疾短螺旋体抗原反应的各种抗血清筛选了三个不同的基因组文库。使用非离子型清洁剂Triton X-114对克隆的抗原和相应的天然猪痢疾短螺旋体抗原进行分子大小，血清反应性，肌氨酸溶解性和相分离过程中的分离分析。这些分析的结果表明，基因产物是猪痢疾螺旋体的细胞质膜，周质或鞭毛的成分。在猪痢疾短螺旋体感染和免疫的CF-1小鼠模型中测试了克隆的抗原作为候选疫苗。腹腔注射含有克隆抗原的大肠杆菌粗提物不能保护小鼠免受攻击。然而，注射有表达内鞭毛抗原的大肠杆菌克隆粗提物的小鼠血清在体外杀死猪痢疾短螺旋体。此克隆的鞭毛内抗原的部分纯化制剂可保护小鼠抵抗猪痢疾短螺旋体的同源血清型（B204）和异源血清型（B234）的口服攻击。这些结果表明，内鞭毛蛋白可用作抗猪痢疾短螺旋体的有效亚单位疫苗。

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