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首页> 外文期刊>Infection and immunity >Molecular epidemiologic analysis of the type A streptococcal exotoxin (erythrogenic toxin) gene (speA) in clinical Streptococcus pyogenes strains.
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Molecular epidemiologic analysis of the type A streptococcal exotoxin (erythrogenic toxin) gene (speA) in clinical Streptococcus pyogenes strains.

机译：临床化脓性链球菌菌株中A型链球菌外毒素基因（speA）的分子流行病学分析。

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摘要

A molecular epidemiology analysis was performed with over 440 clinical isolates of Streptococcus pyogenes obtained from 11 different countries in order to determine the frequency of occurrence of the type A streptococcal exotoxin (erythrogenic toxin) gene (speA) among group A strains. The colony hybridization technique employing a specific internal fragment of the speA gene was used for initial screening, and all positive results were further confirmed by the Southern hybridization technique. Among over 300 general strains obtained from patients with a variety of diseases, except scarlet fever (such as tonsillitis, impetigo, cellulitis, pyoderma, abscess, rheumatic fever, and glomerulonephritis), 15% were found to contain the speA gene. Among a group of 146 strains obtained from individuals described as having scarlet fever, 45% were shown to contain the speA gene. Further analysis of the data indicated that strains with certain M- or T-type surface antigens showed a higher (such as M and T types 1 and 3/13) or lower (such as M2, M12, T4, T5, and T28) tendency to contain the speA gene. No correlation was found between speA content of a strain and the ability to cause a specific disease, although strains possessing the speA gene were more likely to be associated with scarlet fever and rheumatic fever than with other types of disease.
机译：为了确定在A组毒株中A型链球菌外毒素基因（speA）的发生频率，对从11个不同国家获得的440多个化脓链球菌临床分离株进行了分子流行病学分析。使用speA基因特定内部片段的菌落杂交技术被用于初始筛选，所有的阳性结果通过Southern杂交技术得到了进一步证实。从猩红热（扁桃体炎，脓疱病，蜂窝织炎，脓皮病，脓肿，风湿热和肾小球性肾炎）以外的各种疾病患者获得的300多种普通菌株中，发现有15％含有speA基因。从描述为猩红热的个体获得的146株菌株中，有45％含有speA基因。对数据的进一步分析表明，具有某些M型或T型表面抗原的菌株显示较高（如M和T 1和3/13型）或较低（如M2，M12，T4，T5和T28）含有speA基因的趋势。尽管具有speA基因的菌株比其他类型的疾病更可能与猩红热和风湿热有关，但菌株的speA含量与引起特定疾病的能力之间没有相关性。

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