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首页> 外文期刊>Infection and immunity >Altered expression of the Salmonella typhimurium-specific B-cell repertoire in mice chronically treated with antibodies to immunoglobulin D.
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Altered expression of the Salmonella typhimurium-specific B-cell repertoire in mice chronically treated with antibodies to immunoglobulin D.

机译：在用免疫球蛋白D抗体长期治疗的小鼠中鼠伤寒沙门氏菌特异性B细胞库的表达改变。

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摘要

Using a modification of the splenic focus assay, we analyzed the Salmonella typhimurium-specific B-cell repertoire in salmonella-susceptible BALB/c mice. Although these mice normally succumbed to salmonella infection before antibody was produced, they appeared to have splenic S. typhimurium-specific B-cell precursors that could be activated to differentiate and secrete antibody in a manner which was quantitatively and qualitatively identical to that of salmonella-resistant mouse strains. We also analyzed the primary S. typhimurium-specific B-cell repertoire in BALB/c mice that had been chronically treated with antibodies to immunoglobulin D (IgD) and therefore had no surface IgD-positive B cells. Although the frequency of S. typhimurium-specific precursors in these mice was similar to that of control mice, there was an apparent alteration in the isotype distribution pattern in anti-IgD-treated mice. Control mice generated a significantly greater proportion of IgG-secreting clones than did anti-IgD-treated mice. In addition, a greater proportion of S. typhimurium-specific clones from control mice secreted IgG2 than secreted IgG1, and those clones that secreted IgG2 but not IgM, IgG3, or IgG1 were greater than 20-fold more common in control than in anti-IgD-treated mice. Finally, we analyzed the immune response of control and anti-IgD-treated mice to a live avirulent vaccine, S. typhimurium SL3235. Although both groups were protected after challenge with a live virulent S. typhimurium strain, only the control mice made serum antibodies to this vaccine. Taken together, these results show that (i) salmonella-susceptible BALB/c mice have S. typhimurium-specific B cells, (ii) the S. typhimurium-specific B cells in anti-IgD-treated mice may have a restricted capacity to switch heavy-chain classes, (iii) the similarity observed in the frequency of the S. typhimurium-specific precursors for these two groups of BALB/c mice is not reflected in the serum, and (iv) the failure of anti-IgD-treated mice to generate a serum antibody response to SL3235 in the face of complete protection suggests that this model may be used to study cell-mediated immune mechanisms in the apparent absence of humoral immunity.
机译：使用脾脏聚焦分析的修改，我们分析了沙门氏菌敏感的BALB / c小鼠中的鼠伤寒沙门氏菌特异性B细胞组成。尽管这些小鼠通常在产生抗体前就已死于沙门氏菌感染，但它们似乎具有脾炎鼠伤寒沙门氏菌特异的B细胞前体，这些前体可以被激活以分化和分泌抗体，其数量和质量与沙门氏菌相同。抗性小鼠品系。我们还分析了BALB / c小鼠中主要的鼠伤寒沙门氏菌特异性B细胞库，这些小鼠已经用免疫球蛋白D（IgD）抗体进行了长期治疗，因此没有表面IgD阳性B细胞。尽管这些小鼠中鼠伤寒沙门氏菌特异性前体的频率与对照小鼠相似，但在抗IgD处理的小鼠中，同种型分布模式有明显改变。对照小鼠比抗IgD处理的小鼠产生显着更大比例的分泌IgG的克隆。此外，来自对照小鼠的鼠伤寒沙门氏菌特异性克隆分泌的IgG2比分泌的IgG1更大，并且那些分泌IgG2但不分泌IgM，IgG3或IgG1的克隆在对照中的普遍性比抗IgG2高20倍。 IgD处理的小鼠。最后，我们分析了对照和抗IgD处理的小鼠对活无毒疫苗鼠伤寒沙门氏菌SL3235的免疫应答。尽管两组均受到活毒鼠伤寒沙门氏菌的攻击后受到保护，但只有对照小鼠对这种疫苗产生了血清抗体。综上所述，这些结果表明（i）沙门氏菌敏感的BALB / c小鼠具有鼠伤寒沙门氏菌特异性B细胞，（ii）抗IgD治疗的小鼠中的鼠伤寒沙门氏菌特异性B细胞可能具有有限的抗开关重链类别，（iii）这两组BALB / c小鼠在鼠伤寒沙门氏菌特异性前体的频率中观察到的相似性未在血清中反映出来，并且（iv）抗IgD-在完全保护的情况下，经处理的小鼠产生对SL3235的血清抗体应答，表明该模型可用于在明显缺乏体液免疫的情况下研究细胞介导的免疫机制。

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