• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Pseudomonas cepacia adherence to respiratory epithelial cells is enhanced by Pseudomonas aeruginosa.
【24h】

Pseudomonas cepacia adherence to respiratory epithelial cells is enhanced by Pseudomonas aeruginosa.

机译：铜绿假单胞菌增强了洋葱假单胞菌对呼吸道上皮细胞的粘附。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Pseudomonas aeruginosa and Pseudomonas cepacia are both opportunistic pathogens of patients with cystic fibrosis. The binding characteristics of these two species were compared to determine if they use similar mechanisms to adhere to respiratory epithelial cells. P. cepacia 249 was shown to be piliated, but there was no detectable homology between P. aeruginosa pilin gene probes and P. cepacia genomic DNA. P. cepacia and P. aeruginosa did not appear to compete for epithelial receptors. In the presence of purified P. aeruginosa pili, the adherence of 35S-labeled strain 249 to respiratory epithelial monolayers was unaffected, while that of P. aeruginosa PAO1 was decreased by 55%. The binding of P. cepacia 249 and 715j was increased by 2.4-fold and 1.5-fold, respectively, in the presence of an equal inoculum of PAO1. Interbacterial agglutination contributed to the increased adherence of P. cepacia, as the binding of 249 was increased twofold in the presence of irradiated PAO1. PAO1 exoproducts had a marked effect in enhancing the ability of the P. cepacia strains to adhere to the epithelial monolayers. A PAO1 supernatant increased the binding of 249 by eightfold and that of 715j by fourfold. Thus, there appears to be a synergistic relationship between P. aeruginosa and P. cepacia in which PAO1 exoproducts modify the epithelial cell surface, exposing receptors and facilitating increased P. cepacia attachment.
机译：铜绿假单胞菌和洋葱状假单胞菌都是囊性纤维化患者的机会病原体。比较了这两个物种的结合特性，以确定它们是否使用类似的机制粘附于呼吸道上皮细胞。洋葱假单胞菌249显示为有毛的，但铜绿假单胞菌菌毛蛋白基因探针与洋葱假单胞菌基因组DNA之间没有可检测的同源性。洋葱假单胞菌和铜绿假单胞菌似乎没有竞争上皮受体。在纯化的铜绿假单胞菌菌毛的存在下，35S标记的菌株249对呼吸道上皮单层的粘附不受影响，而铜绿假单胞菌PAO1的粘附力降低了55％。在PAO1接种量相同的情况下，洋葱伯克霍尔德菌249和715j的结合分别增加了2.4倍和1.5倍。细菌间的凝集作用导致洋葱伯克霍尔德菌的粘附增加，因为在辐照的PAO1存在下249的结合增加了两倍。 PAO1外生产物在增强洋葱伯克霍尔德氏菌菌株粘附于上皮单层的能力方面具有显著作用。 PAO1上清液将249的结合提高了八倍，而将715j的结合提高了四倍。因此，铜绿假单胞菌和洋葱伯克霍尔德森菌之间似乎存在协同关系，其中PAO1外生产物修饰上皮细胞表面，暴露受体并促进洋葱伯克霍尔伯氏菌附着增加。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号